Evidence map›Paper›PMID 40924373›Full record

ArticleApplied biochemistry and biotechnology2025

Characterization and Development of Salt-Resistant Aspergillus oryzae for Marine-Derived Enzyme Expression.

Bei Han, Qianqian Shao, Yilun Zhang, Qi Liu, Menghao Cai

Abstract read
PubMed Publisher
In one paragraph

Article in Applied biochemistry and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Bei HanState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Qianqian ShaoState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Yilun ZhangState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Qi LiuState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China. liuqi0496@ecust.edu.cn.
Menghao CaiState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China. cmh022199@ecust.edu.cn.ORCID http://orcid.org/0000-0001-6225-8515

Funding

China Postdoctoral Science Foundation 2022M711146National Key R&D Program of China 2022YFC2805102National Natural Science Foundation of China 32201206
6 · The paper itself

Abstract

Marine-derived enzymes often show distinct physiological properties and great potential for industrial use. Salt ions may improve the stability and expression efficiency of marine enzymes, which requires salt-resistant host based expression platform. Aspergillus oryzae of good protein expression and secretion was evaluated and explored for this purpose. Growth, sporulation, secreted extracellular proteins and transcriptome-based metabolic characteristics under artificial seawater condition were analyzed. Also, genetic manipulation system was developed and compared in various strains. Finally, A. oryzae AS 3.487 was recognized as a dominant chassis host. Efficient constitutive and artificial seawater-inducible promoters were screened from the transcriptomic data, which were further evaluated using marine alginate lyase AlgI as the reporter. Medium and condition optimizations improved enzyme production subsequently. Multicopy expression strategy further achieved a maximum enzyme production of 797 U/mL in flask culture. This study provides new references for the heterologous expression of marine-derived enzymes under high-salinity culture conditions.

Indexed as

Aquatic OrganismsAspergillus oryzaeFungal ProteinsPolysaccharide-LyasesSalinitySalt ToleranceSeawaterFungal Proteinspoly(beta-D-mannuronate) lyasePolysaccharide-LyasesAlginate lyaseA. oryzaeHeterologous expressionMarine enzymeTranscriptomic analysis

Identifiers

PMID40924373

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.