Evidence map›Paper›PMID 40922480›Full record

ArticleEuropean journal of haematology2025

Performance of Rapid Clonotyping of Chronic Lymphocytic Leukemia Using Flongle Flow-Cell Nanopore Sequencing of IGH Rearrangements.

Marcus Høy Hansen, Rona Gulmir, Oriane Cédile, Sara Kamuk Dahlmann, Sólja Remisdóttir Veyhe, Magnus Stougaard, Karen Juul-Jensen, Charlotte Guldborg Nyvold

Abstract read
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In one paragraph

Article in European journal of haematology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Marcus Høy HansenHaematology-Pathology Research Laboratory, Research Unit for Haematology and Research Unit for Pathology, University of Southern Denmark and Odense University Hospital, Odense, Denmark.ORCID https://orcid.org/0000-0003-3083-4850
Rona GulmirHaematology-Pathology Research Laboratory, Research Unit for Haematology and Research Unit for Pathology, University of Southern Denmark and Odense University Hospital, Odense, Denmark.
Oriane CédileHaematology-Pathology Research Laboratory, Research Unit for Haematology and Research Unit for Pathology, University of Southern Denmark and Odense University Hospital, Odense, Denmark.ORCID https://orcid.org/0000-0002-6632-8039
Sara Kamuk DahlmannHaematology-Pathology Research Laboratory, Research Unit for Haematology and Research Unit for Pathology, University of Southern Denmark and Odense University Hospital, Odense, Denmark.ORCID https://orcid.org/0000-0002-6427-4276
Sólja Remisdóttir VeyheHaematology-Pathology Research Laboratory, Research Unit for Haematology and Research Unit for Pathology, University of Southern Denmark and Odense University Hospital, Odense, Denmark.ORCID https://orcid.org/0000-0002-5018-3728
Magnus StougaardDepartment for Clinical Genetics, Department of Clinical Medicine, Aarhus University and Aarhus University Hospital, Aarhus, Denmark.ORCID https://orcid.org/0000-0001-7456-902X
Karen Juul-JensenHaematology-Pathology Research Laboratory, Research Unit for Haematology and Research Unit for Pathology, University of Southern Denmark and Odense University Hospital, Odense, Denmark.ORCID https://orcid.org/0000-0003-2158-0107
Charlotte Guldborg NyvoldHaematology-Pathology Research Laboratory, Research Unit for Haematology and Research Unit for Pathology, University of Southern Denmark and Odense University Hospital, Odense, Denmark.ORCID https://orcid.org/0000-0002-0411-7594

Funding

Eva og Henry Frænkels Mindefond 2023-0115
6 · The paper itself

Abstract

backgroundClonotyping of immunoglobulin heavy chain (IGH) gene rearrangements is critical for diagnosis, prognostication, and measurable residual disease monitoring in chronic lymphocytic leukemia (CLL). Although short-read next-generation sequencing (NGS) platforms, such as Illumina MiSeq, are widely used, they face challenges in spanning full VDJ rearrangements. Long-read sequencing via Oxford Nanopore Technologies (ONT) offers a potential alternative using the compact and cost-effective flow cells.

methodsWe evaluated IGH clonotyping in samples from 13 CLL patients using ONT MinION with Flongle flow cells and super-accuracy GPU-based base calling (Dorado), comparing results to MiSeq-based LymphoTrack assays. Amplicons were generated using the IGH FR1 assay and analyzed using Smith-Waterman alignment and IgBlast tools.

resultsAll major and minor clonotypes identified by MiSeq were matched with 100% sequence identity using Nanopore sequencing. Clonal burden estimates were strongly correlated (Pearson ρ = 0.87, p < 10

conclusionNanopore sequencing enables accurate and rapid IGH clonotyping in CLL, offering comparable performance to MiSeq with lower cost and laboratory footprint. This supports its potential utility in routine and decentralized hematopathology workflows.

Indexed as

Gene RearrangementGene Rearrangement, B-Lymphocyte, Heavy ChainImmunoglobulin Heavy ChainsLeukemia, Lymphocytic, Chronic, B-CellNanopore SequencingAgedFemaleFlow CytometryHigh-Throughput Nucleotide SequencingHumansMaleImmunoglobulin Heavy Chainschronic lymphocytic leukemiaclonotype precisionflongle flow cellimmunoglobulin heavy chain clonotypingnanopore sequencingsomatic hypermutation

Identifiers

PMID40922480
PMCPMC12582905

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.