Evidence map›Paper›PMID 40922468›Full record

ArticleMicrobiologyOpen2025

Profiling Listeria monocytogenes in Hummus, Fresh Produce, and Food Processing Environments in the Western Cape, South Africa.

Samantha Anne du Toit, Pieter A Gouws, Diane Rip

Abstract read
In one paragraph

Article in MicrobiologyOpen, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Genetic characterization ofFrontiers in microbiology · 2026
    Article
  2. Microorganisms · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Samantha Anne du ToitDepartment of Food Science, Stellenbosch University, Stellenbosch, Western Cape, South Africa.
Pieter A GouwsDepartment of Food Science, Stellenbosch University, Stellenbosch, Western Cape, South Africa.
Diane RipDepartment of Food Science, Stellenbosch University, Stellenbosch, Western Cape, South Africa.ORCID https://orcid.org/0000-0001-5022-8554

Funding

This study was supported by National Research Foundation South Africa (138101).
6 · The paper itself

Abstract

Listeria monocytogenes is pervasive in agricultural environments and difficult to eradicate from food-processing facilities. Consequently, various foods become contaminated, posing health risks to immunocompromised individuals. This surveillance study aimed to enhance the understanding of the genetic diversity, virulence, plasmid content, sanitizer tolerance, and antibiotic resistance of L. monocytogenes from ready-to-eat (RTE) hummus, fresh produce and food-processing environments in the Western Cape, South Africa (2018-2021). Sixty L. monocytogenes isolates were classified as lineage I or lineage II using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Lineage I was notably prevalent overall (57%; n = 34) and significantly associated with fresh produce (88%; n = 7) (p = 0.04). Whole-genome sequencing (WGS) and bioinformatic analysis characterized a subset of 20 L. monocytogenes isolates into seven sequence types (STs) (ST1, ST2, ST3, ST5, ST101, ST121, ST204), and three serotypes (1/2a, 1/2b, 4b). ST204 (38%; n = 3) was most prevalent in the food-processing environment, ST5 (50%; n = 3) in fresh produce, and ST5 (33%; n = 2), ST101 (33%; n = 2) and ST121 (33%; n = 2), in RTE hummus. However, no single serotype or ST was significantly overrepresented in any category (p > 0.05). All isolates carried both Listeria pathogenicity island (LIPI)-1 and LIPI-2 gene clusters. Two isolates (ST1 and ST3) contained all genes comprising LIPI-3. Two ST5 isolates from RTE hummus contained the tetM gene. Isolates from different origins contained the emrC, bcrABC or qacH genes conferring tolerance to benzalkonium chloride, a quaternary ammonium compound class of sanitizer. RTE hummus, fresh produce and the food-processing environment are susceptible to contamination by diverse and virulent L. monocytogenes strains.

Indexed as

Food MicrobiologyListeria monocytogenesVegetablesAnti-Bacterial AgentsFood HandlingGenetic VariationHumansPlasmidsPolymorphism, Restriction Fragment LengthSouth AfricaVirulenceVirulence FactorsWhole Genome SequencingAnti-Bacterial AgentsVirulence Factorsantibiotic resistancefood safetylisteriosisvirulence

Identifiers

PMID40922468
PMCPMC12417568

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.