ArticleInternational journal of implant dentistry2025
Hyaluronic acid promotes biomineralization of osteoblast-like cells - observations on two different barrier membranes.
Article in International journal of implant dentistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
5 citing papers in PubMed.
- Recreational Allergic Contact Dermatitis to "Epoxy UV" Resins: A New Source of Exposure to Hidden (Meth)acrylates.Contact dermatitis · 2026Article
- Current understanding and future scope of nanotechnology enabled epidermal care using cosmetics and cosmeceuticals.Discover nano · 2026Review
- Hyaluronic Acid-Based Gels and Biomaterial Systems for Oral Wound Healing: Design and Clinical Translation.Gels (Basel, Switzerland) · 2026Review
- Comparing Two Surgical Approaches Using Cross-Linked Hyaluronic Acid-Biofunctionalized Alloplast Particulate in Sinus Floor Elevation: A Randomized Clinical Trial.Journal of functional biomaterials · 2026Article
- RNA-Seq of Gingival Fibroblasts Grown on Collagen Membranes and Hyaluronic Acid.Journal of functional biomaterials · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGuided bone regeneration (GBR) relies on biocompatible membranes to support osteogenesis. 1,4-butanediol diglycidyl ether (BDDE)-crosslinked hyaluronic acid (xHyA) has shown promise in enhancing bone regeneration, yet its mechanisms remain unclear.
objectiveThis study evaluates the osteogenic effects of xHyA-functionalized native pericardium collagen membrane (NPCM) and ribose-crosslinked collagen membrane (RCCM) using an airlift culture model with SaOS-2 cells. Transcriptomic changes following xHyA treatment were also investigated.
methodsSaOS-2 cells were cultured on NPCM and RCCM, with or without xHyA functionalization. Cytocompatibility, alkaline phosphatase (ALP) activity, mineralization (Von Kossa staining), and RNA sequencing were assessed. Differential gene expression and pathway enrichment analyses were conducted on cells exposed to two xHyA concentrations.
resultsBoth membrane types supported cell viability, though NPCM allowed cellular infiltration while RCCM maintained barrier integrity. xHyA significantly enhanced ALP activity and mineral deposition across both substrates. RNA sequencing revealed minimal upregulation of classical osteogenic genes but identified differential expression in pathways related to focal adhesion, VEGF signaling, and stem cell differentiation. IGF1R, FYN, and MAPK14 were consistently upregulated regardless of xHyA concentration.
conclusionxHyA functionalization enhances osteogenic activity, evidenced by increased ALP and mineralization, likely via modulation of cell-matrix interactions rather than classical osteogenic gene activation. These findings support using xHyA-functionalized membranes in GBR and warrant further investigation in vivo.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.