ArticlePlant biotechnology journal2026
Combined Transcriptomic and Metabolomic Analysis Reveals an Ethylene-Activated Regulatory Model on Monoterpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus.
Article in Plant biotechnology journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- HOM2 and TPK1 are associated with Kluyveromyces marxianus growth under high-temperature and reduced-aeration conditions.World journal of microbiology & biotechnology · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
Catharanthus roseus contains nearly 200 bioactive monoterpenoid indole alkaloids (MIAs) that are effective in treating cancer and other diseases. Ethylene plays a significant role in enhancing MIA biosynthesis, and we have found that it greatly induces the accumulation of anhydrovinblastine. However, the regulatory mechanisms underlying this process are not yet fully understood. In this study, a comprehensive analysis of the metabolome and transcriptome of C. roseus was conducted to identify two EIN3/EIL transcription factors, CrEIN3 and CrEIL1, which act as key components mediating the ethylene signal to upregulate MIA biosynthesis. Both CrEIN3 and CrEIL1 were found to upregulate the expression of MIA biosynthetic genes and the activator gene ORCA3, while repressing the expression of repressor genes GBF1 and ZCT1, resulting in increased vinblastine production in C. roseus. CrEIN3 directly binds to the SGD promoter, while CrEIL1 interacts with JA-induced BIS2 to enhance upregulation of the iridoid pathway, thereby further promoting downstream MIA biosynthesis and strengthening the accumulation of bisindole MIAs. Our findings reveal an ethylene-activated regulatory model consisting of CrEIN3 and CrEIL1 that integrates JA-induced BIS2 to cooperatively regulate MIA production in C. roseus, shedding light on the mechanism of ethylene signal regulating MIA biosynthesis. This research provides a foundation for understanding plant hormone regulation of alkaloid metabolism, which will contribute to future efforts in developing high-yielding MIAs in plant or yeast-based platforms.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.