Evidence map›Paper›PMID 40920075›Full record

ArticleeLife2025

Sld3CBD-Cdc45 structural insights into Cdc45 recruitment for CMG complex formation during DNA replication.

Hao Li, Izumi Ishizaki, Koji Kato, Xiaomei Sun, Sachiko Muramatsu, Hiroshi Itou, Toyoyuki Ose, Hiroyuki Araki, Min Yao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in eLife, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Hao LiGraduate School of Life Science, Hokkaido University, Sapporo, Japan.ORCID https://orcid.org/0000-0001-7885-9841
Izumi IshizakiGraduate School of Life Science, Hokkaido University, Sapporo, Japan.
Koji KatoGraduate School of Life Science, Hokkaido University, Sapporo, Japan.
Xiaomei SunFaculty of Advanced Life Science, Hokkaido University, Sapporo, Japan.
Sachiko MuramatsuNational Institute of Genetics, Mishima, Japan.
Hiroshi ItouNational Institute of Genetics, Mishima, Japan.
Toyoyuki OseGraduate School of Life Science, Hokkaido University, Sapporo, Japan.
Hiroyuki ArakiNational Institute of Genetics, Mishima, Japan.ORCID https://orcid.org/0000-0001-5405-0677
Min YaoGraduate School of Life Science, Hokkaido University, Sapporo, Japan.ORCID https://orcid.org/0000-0003-1687-5904

Funding

Japan Agency for Medical Research and Development, Platform Project for Supporting Drug Discovery and Life Science Research JP18am0101071Japan Agency for Medical Research and Development, Platform Project for Supporting Drug Discovery and Life Science Research JP19am0101083Japan Society for the Promotion of Science, KAKENHI Grant-in-aid for Scientific Research(B) 21H01754
6 · The paper itself

Abstract

DNA replication requires recruitment of Cdc45 and GINS into the MCM double hexamer by initiation factors to form an active helicase, the Cdc45-MCM-GINS (CMG) complex, at the replication origins. The initiation factor Sld3 is a central regulator of Cdc45 and GINS recruitment, working with Sld7 together. However, the mechanism through which Sld3 regulates CMG complex formation remains unclear. Here, we present the structure of the Sld3 Cdc45-binding domain in complex with Cdc45 (Sld3CBD-Cdc45), showing detailed interactions and conformational changes required for binding to each other. The mutant analysis indicated that the binding between Sld3CBD and Cdc45 could be broken easily. We also revealed that Sld3CBD, GINS, and MCM bind to different sites on Cdc45 in the Sld3CBD-CMG model, indicating that after recruitment of Cdc45, Sld7-Sld3 could remain in Cdc45-MCM until CMG formation. The consistency between the particle size of Sld7-Sld3-Cdc45 and the distance between Sld3CBDs in the Cdc45-MCM dimer indicated the binding manner of the Cdc45-Sld3-[Sld7]

Indexed as

Cell Cycle ProteinsDNA-Binding ProteinsDNA ReplicationSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsModels, MolecularNuclear ProteinsProtein BindingProtein ConformationCDC45 protein, S cerevisiaeCell Cycle ProteinsDNA-Binding ProteinsNuclear ProteinsSaccharomyces cerevisiae ProteinsSld3 protein, S cerevisiaebudding yeastDNA replication initiationE. colieukaryotesinitiation factor Sld3K. marxianusmolecular biophysicsrecruiting Cdc45S. cerevisiaestructural biology

Identifiers

PMID40920075
PMCPMC12416888

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.