Evidence map›Paper›PMID 40920009›Full record

ArticleMicrobiology spectrum2025

Ultra-high field strength electroporation enables efficient DNA transformation and genome editing in nontuberculous mycobacteria.

Daoyan Tang, Minggui Wang, Dan Wang, Danni Yang, Yi Cai, Tao Luo, Jianqing He, Qinglan Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Leucine auxotrophic marker for genetic manipulation ofApplied and environmental microbiology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Daoyan Tang *Department of Respiratory and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu, China.ORCID 0009-0007-6215-4055
Minggui Wang *Institute of Respiratory Health, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, China.
Dan WangInstitute of Respiratory Health, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, China.
Danni YangInstitute of Respiratory Health, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, China.
Yi CaiGuangdong Provincial Key Laboratory of Infection Immunity and Inflammation, Department of Pathogen Biology, Shenzhen University Medical School, Shenzhen, China.ORCID 0000-0002-1363-2328
Tao LuoDepartment of Pathogen Biology, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, China.ORCID 0000-0002-2929-2233
Jianqing HeDepartment of Respiratory and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu, China.ORCID 0000-0002-4214-2037
Qinglan WangInstitute of Respiratory Health, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, China.ORCID 0000-0002-7629-5058

Funding

National Natural Science Foundation of China No. 82272375Noncommunicable Chronic Diseases-National Science and Technology Major Project of China No. 2024ZD0528400
6 · The paper itself

Abstract

Efficient DNA delivery is essential for genetic manipulation of mycobacteria and for dissecting their physiology, pathogenesis, and drug resistance. Although electroporation enables transformation efficiencies exceeding 10⁵ CFU per µg DNA in

Indexed as

ElectroporationGene EditingNontuberculous MycobacteriaTransformation, BacterialDNA, BacterialGenome, BacterialMycobacterium abscessusMycobacterium marinumPlasmidsDNA, BacterialDNA transformationgenome editingMycobacterium abscessusnontuberculous mycobacteriaUltra-high field strength electroporation

Identifiers

PMID40920009
PMCPMC12502714

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.