Evidence map›Paper›PMID 40919935›Full record

ArticleJournal of bacteriology2025

Analysis of essential genes in

Maia E Alberts, Micaila P Kurtz, Ute Müh, Jonathon P Bernardi, Kevin W Bollinger, Horia A Dobrila, Leonard Duncan, Hannah M Laster, Andres J Orea, Anthony G Pannullo and 4 more

Abstract read
In one paragraph

Article in Journal of bacteriology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Defining the order of assembly of theJournal of bacteriology · 2026
    Article
  2. Article
  3. Article
  4. RNAP-seq:bioRxiv : the preprint server for biology · 2026
    Article
  5. CenIR, an essential BlaIR-family regulatory system inbioRxiv : the preprint server for biology · 2026
    Article
  6. Article
  7. Defining the order of assembly of thebioRxiv : the preprint server for biology · 2026
    Article
  8. Review
  9. Article
  10. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors.

Maia E Alberts *Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
Micaila P Kurtz *Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
Ute Müh *Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.ORCID 0009-0007-7114-019X
Jonathon P BernardiDepartment of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
Kevin W BollingerDepartment of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
Horia A DobrilaDepartment of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.ORCID 0009-0001-1530-4301
Leonard DuncanDepartment of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
Hannah M LasterDepartment of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
Andres J OreaDepartment of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
Anthony G PannulloDepartment of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
Juan G Rivera-RosadoDepartment of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
Facundo V TorresDepartment of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.
Craig D EllermeierDepartment of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.ORCID 0000-0002-8953-5081
David S WeissDepartment of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA.ORCID 0000-0003-2217-0070

Funding

Cell Envelope Biogenesis in Clostridioides difficileR01AI155492 · NIAID · UNIVERSITY OF IOWA · PI ELLERMEIER, CRAIG D, WEISS, DAVID S · 2021 to 2025
$2.6M
CRISPR interference-enabled phenotyping of essential genes in C. difficile to aid in discovery of antibiotic targetsR21AI159071 · NIAID · UNIVERSITY OF IOWA · PI WEISS, DAVID S · 2022 to 2023
$382k
National Science Foundation DBI-1852070NIAID NIH HHS R01 AI155492NIAID NIH HHS R21 AI159071NSF DBI-2244169
6 · The paper itself

Abstract

Essential genes are interesting in their own right and as potential antibiotic targets. To date, only one report has identified essential genes on a genome-wide scale in

Indexed as

Clostridioides difficileClustered Regularly Interspaced Short Palindromic RepeatsDNA Transposable ElementsGenes, EssentialBacterial ProteinsCRISPR-Cas SystemsMutagenesis, InsertionalBacterial ProteinsDNA Transposable ElementsCRISPRiTn-seq

Identifiers

PMID40919935
PMCPMC12548448

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.