Pathogen reduction diminishes the protective effects of platelets on endothelial barrier permeability in vitro.

Alison B Nair, Byron Miyazawa, Alpa Trivedi, Lindsay Vivona, Alexander Fields, Kimberly Herrera, Lucy Z Kornblith, Joseph Cuschieri, Shibani Pati

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Article in Blood vessels, thrombosis & hemostasis, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Alison B NairDepartment of Pediatrics, University of California, San Francisco, CA.

Byron MiyazawaDepartment of Laboratory Medicine, University of California, San Francisco, CA.

Alpa TrivediDepartment of Laboratory Medicine, University of California, San Francisco, CA.

Lindsay VivonaDepartment of Laboratory Medicine, University of California, San Francisco, CA.

Alexander FieldsDepartment of Surgery, University of California, San Francisco, CA.

Kimberly HerreraDepartment of Surgery, University of California, San Francisco, CA.

Lucy Z KornblithDepartment of Laboratory Medicine, University of California, San Francisco, CA.

Joseph CuschieriDepartment of Laboratory Medicine, University of California, San Francisco, CA.

Shibani PatiDepartment of Laboratory Medicine, University of California, San Francisco, CA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Platelet transfusion not only attenuates bleeding and promotes hemostasis but also plays a critical role in vascular stability and endothelial barrier integrity. Under amotosalen-UVA pathogen reduction of platelets, pathogen nucleic acids undergo adduction, which prevents their replication and greatly reduces the risk of transfusion-transmitted infections. Although pathogen-reduced (PR) platelets are increasing in clinical use, the physiologic effects of pathogen reduction on platelets, particularly its impact on platelet-endothelial interactions, have yet to be described. This study compared PR platelets with nonpathogen-reduced (NPR) platelets in measures of effect on endothelial permeability in vitro. We hypothesized that PR platelets would be similar to NPR platelets. However, in endothelial cell immunohistochemistry and impedance assays, PR platelets demonstrated a significantly diminished capacity to attenuate endothelial barrier permeability at early storage time points. This small but significant difference requires further mechanistic and clinical study to understand its implications, particularly in patients with bleeding with vascular fragility.

Identifiers

PMID40918743

DOI10.1016/j.bvth.2025.100058

PMCPMC12412405

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LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.