ArticleiScience2025
OpenEMMU: A versatile, open-source EdU multiplexing methodology for studying DNA replication and cell cycle dynamics.
Article in iScience, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Big1 is a cell-cycle regulator linking cell size to basal body number in Tetrahymena thermophila.Current biology : CB · 2026Article
- Multi-omic analysis of human PHACTR1 signaling networks.Communications biology · 2026Article
- A Scalable MNase-seq Framework for Reproducible Nucleosome Profiling across Pluripotent Stem Cell and Cardiomyocyte Models.Computational and structural biotechnology journal · 2026Article
- Single-cell RNA sequencing revealed cell heterogeneity in sagittal suture mesenchyme.Frontiers in cell and developmental biology · 2026Article
- Big1 is a cell cycle regulator linking cell size to basal body number.bioRxiv : the preprint server for biology · 2025Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
5-Ethynyl-2'-deoxyuridine (EdU) has revolutionized DNA replication and cell cycle analyses through fast, efficient click chemistry detection. However, commercial EdU kits suffer from high costs, proprietary formulations, limited antibody multiplexing capabilities, and difficulties with larger biological specimens. Here, we present OpenEMMU (Open-source EdU Multiplexing Methodology for Understanding DNA replication dynamics), an optimized, affordable, and user-friendly click chemistry platform utilizing off-the-shelf reagents. OpenEMMU enhances efficiency, brightness, and multiplexing capabilities of EdU staining with both non-conjugated and conjugated antibodies across diverse cell types, including T cell activation and proliferation assays. We validated its effectiveness for the fluorescent imaging of nascent DNA synthesis in developing embryos and organs, including embryonic heart, forelimbs, and 3D hiPSC-derived cardiac organoids. OpenEMMU also enabled the deep-tissue 3D imaging of DNA synthesis in zebrafish larvae and under replication stress in embryos at high spatial resolution. This approach opens new avenues for understanding organismal development, cell proliferation, and DNA replication dynamics with unprecedented precision and flexibility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.