Evidence map›Paper›PMID 40914935›Full record

ArticleSTAR protocols2025

Protocol for live-cell calcium imaging of human lung microvascular endothelial vessel-on-a-chip model.

Nethika Ariyasinghe, Jean-Pierre Amoakon, Anjaparavanda P Naren

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Nethika AriyasingheDivision of Pulmonary Medicine and Critical Care, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA. Electronic address: nethika.ariyasinghe@cshs.org.
Jean-Pierre AmoakonDivision of Pulmonary Medicine and Critical Care, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Department of Systems Biology and Physiology, University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA. Electronic address: amoakoja@mail.uc.edu.
Anjaparavanda P NarenDivision of Pulmonary Medicine and Critical Care, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Department of Systems Biology and Physiology, University of Cincinnati College of Medicine, Cincinnati, OH 45229, USA; Department of Pulmonary Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA; Division of Gastroenterology and Hepatology, Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Calcium signaling is crucial for endothelial cell homeostasis. Alterations in intracellular calcium levels due to shear stress are linked to vascular dysfunction and diseases. Here, we present a protocol to perform live calcium imaging by using a live calcium indicator on human lung endothelial cells subjected to shear stress in a commercially available microfluidic device (Ibidi Luer VI). We also outline data analysis procedures. This protocol can test altered shear stress effects in the vasculature and identify potential drug treatments. For complete information on the generation and use of this protocol, please refer to Amoakon et al.

Indexed as

CalciumEndothelial CellsLab-On-A-Chip DevicesLungMicrovesselsCalcium SignalingHumansCalciumBiotechnology and bioengineeringCell-based AssaysTissue Engineering

Identifiers

PMID40914935
PMCPMC12451350

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.