Evidence map›Paper›PMID 40914787›Full record

ArticleDrug delivery and translational research2025

Hanging drop culture reprograms mesenchymal stem cell transcriptome to enhance cell delivery efficiency via attenuated pulmonary entrapment.

Hsiang-Tzu Lee, Sheng-Yung Fu, Wei-Han Weng, Wei-Chen Chao, Yu-Pao Hsu, Nan-Ping Yang, Yu-Hsu Chen, Shau-Kwaun Chen, Chien-Wen Chang

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Article in Drug delivery and translational research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Hsiang-Tzu LeeDepartment of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, 300044, Taiwan.
Sheng-Yung FuDepartment of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, 300044, Taiwan.
Wei-Han WengDepartment of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, 300044, Taiwan.
Wei-Chen ChaoDepartment of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, 300044, Taiwan.
Yu-Pao HsuDepartment of Orthopedic Surgery, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, Taiwan.
Nan-Ping YangDepartment of Orthopedic Surgery, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, Taiwan.
Yu-Hsu ChenDepartment of Orthopedic Surgery, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, Taiwan. magister.yuhsu@gmail.com.
Shau-Kwaun ChenInstitute of Neuroscience, National Chengchi University, Taipei, Taiwan. chensk@nccu.edu.tw.
Chien-Wen ChangDepartment of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, 300044, Taiwan. chienwen@mx.nthu.edu.tw.ORCID 0000-0002-0701-876X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The three-dimensional (3D) culture system has emerged as an indispensable platform for modulating stem cell function in biomedicine, drug screening, and cell therapy. Despite a few studies confirming the functionality of 3D culture, the molecular factors underlying this process remain obscure. Here, we have utilized a hanging drop method to generate 3D spheroid-derived mesenchymal stem cells (3D MSCs) and compared them to conventionally 2D-cultured MSCs. The results showed that 3D MSCs exhibited distinct phenotypic features than 2D-cultured MSCs and expressed different transcriptional responses, as found from RNA-Seq analysis. Gene ontology (GO) annotations and KEGG pathway mapping pinpointed that the 3D MSCs responded more actively to incoming signals as they upregulated receptors and cytokine production while downregulating proteolysis-, cytoskeletal-, extracellular matrix-, and adhesion-related genes. Functionally, these MSCs also displayed enhanced chemotaxis and improved pulmonary transgression post-intravenous injection. This study provides mechanistic insights on addressing a significant limitation of MSC therapy: pulmonary entrapment after systemic delivery. Moreover, upregulated pluripotency-associated genes, such as Oct4, Sox2, and Nanog, suggest that the 3D MSCs possessed enhanced stemness and regenerative capacity. The results indicate that 3D spheroid culture reshapes MSC transcriptomic and functional profiles and emerges as a promising strategy for improving their therapeutic potential in regenerative medicine.

Indexed as

Mesenchymal Stem CellsMesenchymal Stem Cell TransplantationTranscriptomeAnimalsCell Culture Techniques, Three DimensionalCells, CulturedHumansLungMaleSpheroids, CellularChemotaxisHanging drop methodLung trappingMesenchymal stem cellThree-dimensional cell culture

Identifiers

PMID40914787
PMCPMC12507987

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.