Evidence map›Paper›PMID 40913702›Full record

ArticleApplied biochemistry and biotechnology2025

Heterologous Expression, Purification, and Characterization of a High-Temperature Requirement A (HtrA) Protease Homologue from Leptospira.

Preeti Kumari, Garima Singh, Samarendra K Singh, Padikara K Satheeshkumar

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Article in Applied biochemistry and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Preeti KumariDepartment of Botany, Institute of Science, Banaras Hindu University, Varanasi, Uttar Pradesh, 221005, India.
Garima SinghSchool of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi, Uttar Pradesh, 221005, India.
Samarendra K SinghSchool of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi, Uttar Pradesh, 221005, India.
Padikara K SatheeshkumarDepartment of Botany, Institute of Science, Banaras Hindu University, Varanasi, Uttar Pradesh, 221005, India. satheesh.bot@bhu.ac.in.

Funding

Council of Scientific and Industrial Research, India 09/013(0884)/2019-EMR-IIoE, Banaras Hindu University R/Dev/D/loE/incentive/2022-23/47678IoE, Banaras Hindu University R/Dev/D/loE/TDR(Phase-II)/2023-24/68189Science and Engineering Research Board (SERB), Govt. of India EEQ/2018/000254
6 · The paper itself

Abstract

Leptospirosis is a zoonotic disease affecting humans in the tropical and temperate regions. Considerably high mortality rate (60 per 1000 adult) and associated morbidity necessitate the need for efficient diagnostic and therapeutic approaches for this disease. Proteins that play crucial roles in the invasion/pathogenesis are potential candidates for the diagnosis/therapeutics. High temperature requirement A (HtrA) is a protein expressed by many pathogenic bacteria, important for their virulence and survival. In this study, we have amplified, cloned, and expressed one of the HtrA homologues (HtrA1) from Leptospira. The expressed recombinant HtrA was purified using Ni-NTA chromatography. Physicochemical characterization of the enzyme using azo-casein substrate showed the maximum activity at a temperature 42 °C and pH 7. While Mn

Indexed as

Bacterial ProteinsHigh-Temperature Requirement A Serine Peptidase 1LeptospiraAmino Acid SequenceCell Line, TumorCloning, MolecularHumansHydrogen-Ion ConcentrationProtein BindingRecombinant ProteinsTemperatureBacterial ProteinsHigh-Temperature Requirement A Serine Peptidase 1Recombinant ProteinsCell-junction cleavageLeptospirosisPathogen invasionVirulence gene

Identifiers

PMID40913702

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