Evidence map›Paper›PMID 40913202›Full record

ArticleReproductive sciences (Thousand Oaks, Calif.)2025

C/EBP-β/MeCP2/Wnt Axis Participates in the Testicular Injury in Rats Under Simulated Microgravity Conditions.

Pan Ge, Si-Yu Wang, Xiang Zhang, Yan-Qi Yang, Mo-Qi Lv, Ying Sun, Jian Zhang, Xiao-Ting Wang, Ming Liu, Dang-Xia Zhou

Abstract read
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In one paragraph

Article in Reproductive sciences (Thousand Oaks, Calif.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Pan GeDepartment of Pathology, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.
Si-Yu WangDepartment of Medicine, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.
Xiang ZhangDepartment of Electrocardiographic Diagnosis, Xi'an Children's Hospital, Xi'an, 710003, Shaanxi, China.
Yan-Qi YangDepartment of Pathology, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.
Mo-Qi LvDepartment of Pathology, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.
Ying SunDepartment of Pathology, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.
Jian ZhangDepartment of Pathology, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China.
Xiao-Ting WangReproductive Medicine Center, Xi'an People's Hospital (Xi'an Fourth Hospital), Xi'an, 710004, Shaanxi, China. 369542541@qq.com.
Ming LiuDepartment of Gynecology and Obstetrics, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710001, China. gynliu88@163.com.
Dang-Xia ZhouDepartment of Pathology, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, China. zdxtougao@163.com.ORCID 0000-0002-7072-3170

Funding

National Natural Science Foundation of China 81273018National Natural Science Foundation of China 81673224National Training Program of Innovation and Entrepreneurship for Undergraduates SJ202210698250Natural Science Foundation of Shaanxi Province 2024JC-YBQN-0771projects of educational reform in XJTU 22SJZX46projects of educational reform in XJTU 24ZK39YSchool Enterprise Joint Project of Xi'an Jiaotong University 20201065-ZKT02the Innovation Project for Medical Integration in XJTU YXJLRH2022080
6 · The paper itself

Abstract

BACKGROUND AND

objectiveAs space exploration advances, the effects of the microgravity environment on testicular injury and spermatogenic function in astronauts have attracted widespread attention, but the underlying mechanisms remain unclear.

methodsIn this study, testicular morphometry and Johnsen score were used to evaluate the degree of testicular injury. Then the upstream transcription factors of MeCP2 were verified using the dual-luciferase reporter assay. Finally, the expression of C/EBP-β, MeCP2, and Wnt4/β-Catenin protein was detected to clarify the regulatory pathway.

resultsUnder microgravity conditions, MeCP2 expression was significantly reduced in rat testes and was positively correlated with testicular morphometric indices. Subsequently, the dual-luciferase reporter assay revealed that C/EBP-β directly bound to the promoter sequence of MeCP2, initiating its transcriptional activity. Meanwhile, the expression of C/EBP-β, Wnt4, and β-Catenin was reduced in the testes under microgravity conditions.

conclusionsIn conclusion, our study proposed that C/EBP-β regulated MeCP2, consequently, inhibited the Wnt4/β-Catenin pathway activity, thereby participating in microgravity-induced testicular injury.

Indexed as

CCAAT-Enhancer-Binding Protein-betaMethyl-CpG-Binding Protein 2TestisWeightlessness SimulationWnt4 ProteinWnt Signaling PathwayAnimalsbeta CateninMaleRatsRats, Sprague-Dawleybeta CateninCCAAT-Enhancer-Binding Protein-betaCebpb protein, ratCtnnb1 protein, ratMethyl-CpG-Binding Protein 2Wnt4 ProteinC/EBP-βMeCP2MicrogravityTesticular injuryWnt4/β-Catenin pathway

Identifiers

PMID40913202

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.