Evidence map›Paper›PMID 40909753›Full record

ArticlebioRxiv : the preprint server for biology2025

Endocytome profiling uncovers cell-surface protein dynamics underlying neuronal connectivity.

Colleen N McLaughlin, Hui Ji, Katherine X Dong, Chuanyun Xu, Kenneth Kin Lam Wong, Zhuoran Li, David J Luginbuhl, Charles Xu, Cheng Lyu, Wei Qin and 5 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

15 authors.

Colleen N McLaughlinDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Hui JiDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Katherine X DongDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Chuanyun XuDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Kenneth Kin Lam WongDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Zhuoran LiDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
David J LuginbuhlDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Charles XuThe Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
Cheng LyuDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Wei QinDepartment of Genetics, Biology, and Chemistry, Chan Zuckerberg Biohub, Stanford University, Stanford, CA 94305, USA.
Jiefu LiJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
Namrata D UdeshiThe Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
Steven A CarrThe Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
Alice Y TingDepartment of Genetics, Biology, and Chemistry, Chan Zuckerberg Biohub, Stanford University, Stanford, CA 94305, USA.
Liqun LuoDepartment of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.

Funding

Assembly of the Central Olfactory Networks in DrosophilaR01DC005982 · NIDCD · STANFORD UNIVERSITY · PI LIQUN LUO · 2003 to 2026
$8.1M
Cell surface protein dynamics in neural circuit assemblyK99DC021195 · NIDCD · STANFORD UNIVERSITY · PI MCLAUGHLIN, COLLEEN · 2024 to 2025
$257k
NIDCD NIH HHS K99 DC021195NIDCD NIH HHS R01 DC005982
6 · The paper itself

Abstract

Endocytosis actively remodels the neuronal surface proteome to drive diverse cellular processes, yet its global extent and circuit-level consequences have defied comprehensive interrogation. Here, we introduce endocytome profiling: a systematic, cell-type-specific approach for mapping cell-surface protein (CSP) dynamics in situ. Quantitative proteomic analysis of developing olfactory receptor neuron (ORN) axons generated an endocytic atlas comprising over 1,100 proteins and revealed the extent to which the surface proteome is remodeled to meet distinct developmental demands. Targeted interrogation of a junctional CSP showed that its endosome-to-surface ratio is precisely balanced to enable developmental axon pruning while preserving mature axon integrity. Multi-omic integration uncovered wide-spread transcellular signaling and identified a growth factor secreted by neighboring neurons to direct ORN axon targeting via endocytic regulation of its receptor. Endocytome profiling thus provides unprecedented access to cell-surface proteome dynamics and offers a powerful platform for dissecting proteome remodeling across diverse cell types and contexts.

Identifiers

PMID40909753
PMCPMC12407874

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.