ArticlebioRxiv : the preprint server for biology2025
5' cap sequestration is required for sensing of unspliced HIV-1 RNA by MDA5.
Ivy K Hughes, Siarhei Kharytonchyk, Sita Ramaswamy, Sallieu Jalloh, James B Hood, Xianbao He, Andrew J Henderson, Hisashi Akiyama, Alice Telesnitsky, Suryaram Gummuluru
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In one paragraphArticle in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 · Who and what moneyAuthors and funding
10 authors.
Ivy K HughesDepartment of Virology, Immunology, & Microbiology, Boston University Chobanian & Avedisian School of Medicine.
Siarhei KharytonchykDepartment of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor.
Sita RamaswamyDepartment of Virology, Immunology, & Microbiology, Boston University Chobanian & Avedisian School of Medicine.
Sallieu JallohDepartment of Virology, Immunology, & Microbiology, Boston University Chobanian & Avedisian School of Medicine.
James B HoodDepartment of Virology, Immunology, & Microbiology, Boston University Chobanian & Avedisian School of Medicine.
Xianbao HeDepartment of Medicine, Section of Infectious Diseases, Boston Medical Center.
Andrew J HendersonDepartment of Virology, Immunology, & Microbiology, Boston University Chobanian & Avedisian School of Medicine.
Hisashi AkiyamaDepartment of Virology, Immunology, & Microbiology, Boston University Chobanian & Avedisian School of Medicine.ORCID 0000-0001-9228-0465 Alice TelesnitskyDepartment of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor.ORCID 0000-0001-9878-5931 Suryaram GummuluruDepartment of Virology, Immunology, & Microbiology, Boston University Chobanian & Avedisian School of Medicine.ORCID 0000-0002-8606-8481 Funding
Translational ScienceP30AI042853 · NIAID · MIRIAM HOSPITAL · PI CURT G BECKWITH, DEBBIE M. CHENG · 1998 to 2026
$51.7MCenter for Structural Biology of HIV RNAU54AI170660 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI ALICE TELESNITSKY · 2022 to 2026
$32.1MPersistent HIV-1 expression and microglia dysfunctionR01DA055488 · NIDA · BOSTON MEDICAL CENTER · PI GUMMULURU, SURYARAM, HENDERSON, ANDREW J · 2021 to 2025
$3.7MSynergistic Mechanisms of chronic Innate Immune Activation in Microglia by Opiates and HIV InfectionR01DA051889 · NIDA · BOSTON UNIVERSITY MEDICAL CAMPUS · PI CHENG, CHRISTINE, GUMMULURU, SURYARAM · 2020 to 2024
$3.5MDefective HIV proviruses and Persistent Innate Immune ActivationR01AI187175 · NIAID · BOSTON MEDICAL CENTER · PI SURYARAM GUMMULURU, Andrew J Henderson · 2024 to 2026
$2.4MMechanisms of Persistent Inflammasome Activation in Myeloid CellsR01DA059952 · NIDA · BOSTON UNIVERSITY MEDICAL CAMPUS · PI SURYARAM GUMMULURU, Andrew J Henderson · 2024 to 2026
$2.3MNIAID NIH HHS P30 AI042853NIAID NIH HHS R01 AI187175NIAID NIH HHS U54 AI170660NIDA NIH HHS R01 DA051889NIDA NIH HHS R01 DA055488NIDA NIH HHS R01 DA059952
6 · The paper itselfAbstract
Heterogenous transcription start site (TSS) usage dictates the structure and function of unspliced HIV-1 RNAs (usRNA). We and others have previously reported that expression and Rev/CRM1-mediated nuclear export of HIV-1 usRNA in macrophages activates MDA5, MAVS, and innate immune signaling cascades. In this study, we reveal that MDA5 sensing of viral usRNA is strictly determined by TSS, 5' leader structure, and RNA function. We show that cap-sequestered HIV-1 usRNAs (
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PMID40909469
PMCPMC12407712
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