ArticlePloS one2025
Enhanced electrophysiological recordings in acute brain slices, spheroids, and organoids using 3D high-density multielectrode arrays.
Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- CASCADE: criticality avalanche spike cross-platform analysis detection engine, a multi-manufacturer MEA bash analysis pipeline.Bioinformatics (Oxford, England) · 2026Article
- Measuring Electrophysiological Activity in Acute Brain Slices, Spheroids, and Organoids Using 3D High-Density Multielectrode Arrays.Bio-protocol · 2026Article
- AI-Resolved Protein Energy Landscapes, Electrodynamics, and Fluidic Microcircuits as a Unified Framework for Predicting Neurodegeneration.International journal of molecular sciences · 2026Review
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Authors and funding
21 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Recent advances in three-dimensional (3D) biological brain models in vitro and ex vivo are creating new opportunities to understand the complexity of neural networks but pose the technological challenge of obtaining high-throughput recordings of electrical activity from multiple sites in 3D at high spatiotemporal resolution. This cannot be achieved using planar multi-electrode arrays (MEAs), which contact just one side of the neural structure. Moreover, the specimen adhesion to planar MEAs limits fluid perfusion along with tissue viability and drug application. Here, the efficiency of the tissue-sensor interface provided by advanced 3D high-density (HD)-MEA technology was evaluated in acute brain slices, spheroids, and organoids obtained from different brain regions. The 3D HD-MEA microneedles reached the inner layers of samples without damaging network integrity and the microchannel network between microneedles improved tissue vitality and chemical compound diffusion. In acute cortico-hippocampal and cerebellar slices, signal recording and stimulation efficiency proved higher with the 3D HD-MEA than with a planar MEA improving the characterization of network activity and functional connectivity. The 3D HD-MEA also resolved the challenge of recording from brain spheroids as well as cortical and spinal organoids. Our results show that 3D HD-MEA technology represents a valuable tool to address the complex spatiotemporal organization of activity in brain microcircuits, making it possible to investigate 3D biological models.
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