Evidence map›Paper›PMID 40906331›Full record

ArticleTissue engineering and regenerative medicine2025

Two is Better than One: Evaluation of Single Versus Staged Approaches for Tubular Substitution Urethroplasty with Acellular Silk Fibroin Biomaterials in Rabbits.

Gokhan Gundogdu, David W Barham, Madison Rivero, Travis Nguyen, Charlotte Morgan, Joel Gelman, Joshua R Mauney

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Tissue engineering and regenerative medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Gokhan GundogduDepartment of Urology, University of California, Irvine, 101 The City Drive South., Building 55, Rm. 300, Orange, CA, 92868, USA.
David W BarhamDepartment of Urology, University of California, Irvine, 101 The City Drive South., Building 55, Rm. 300, Orange, CA, 92868, USA.
Madison RiveroDepartment of Biomedical Engineering, University of California, Irvine, Irvine, CA, 92617, USA.
Travis NguyenDepartment of Biomedical Engineering, University of California, Irvine, Irvine, CA, 92617, USA.
Charlotte MorganDepartment of Urology, University of California, Irvine, 101 The City Drive South., Building 55, Rm. 300, Orange, CA, 92868, USA.
Joel GelmanDepartment of Urology, University of California, Irvine, 101 The City Drive South., Building 55, Rm. 300, Orange, CA, 92868, USA.
Joshua R MauneyDepartment of Urology, University of California, Irvine, 101 The City Drive South., Building 55, Rm. 300, Orange, CA, 92868, USA. mauneyj@hs.uci.edu.ORCID http://orcid.org/0000-0002-3186-3664

Funding

Translational Bioreactors - Engineering Complex TissuesP41EB002520 · NIBIB · TUFTS UNIVERSITY MEDFORD · PI KAPLAN, DAVID L. · 2004 to 2018
$16.7M
Drug Eluting Silk Fibroin Grafts for Repair of Long Urethral StricturesR01DK131211 · NIDDK · UNIVERSITY OF CALIFORNIA-IRVINE · PI Joshua Robert Mauney · 2023 to 2026
$2.5M
NIBIB NIH HHS P41 EB002520NIDDK NIH HHS R01 DK131211NIDDK NIH HHS R01DK131211
6 · The paper itself

Abstract

backgroundAcellular bi-layer silk fibroin (BLSF) scaffolds represent potential alternatives to autologous tissue grafts for substitution urethroplasty (SU) given their ability to repair focal urethral defects in animal models. However, in patients with a severe fibrotic urethral plate, single or staged SU are often required to restore organ continuity. Currently, the feasibility of tubular BLSF grafts for urethral replacement is unknown. Therefore, the objective of this study was to evaluate the efficacy of BLSF biomaterials for SU using single and staged approaches.

methodsSingle (N = 4) and staged (N = 5) SU with BLSF grafts were carried out in adult male rabbits, and animals were maintained for 3 months. Nonsurgical control animals (NSC, N = 3) were evaluated in parallel.

resultsAll rabbits survived until harvest and displayed voluntary voiding after initial catheterization with no evidence of severe complications. At 3 months, retrograde urethrograms revealed relative urethral calibers treated with both single and staged approaches were restored to 80 ± 26% and 129 ± 27% of NSC levels. In addition, staged SU led to significantly higher degrees of scaffold degradation as well as urethral patency in respect to the single stage repairs. Histological and immunohistochemical evaluations demonstrated that both surgical techniques supported the formation of innervated, vascularized neotissues resembling NSC. However, neotissues from single stage repairs presented with elevated levels of fibrosis and reduced smooth muscle relative to NSC and the staged cohort.

conclusionsSingle and staged SU with BLSF grafts are feasible for tubular urethral replacement, but staged reconstruction results in improved functional tissue regeneration.

Indexed as

Biocompatible MaterialsFibroinsPlastic Surgery ProceduresUrethraAnimalsMaleRabbitsTissue ScaffoldsBiocompatible MaterialsFibroinsBiomaterialSilk fibroinUrethroplasty

Identifiers

PMID40906331
PMCPMC12476340

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.