ArticleJournal of extracellular vesicles2025
A Compendium of Bona Fide Reference Markers for Genuine Plant Extracellular Vesicles and Their Degree of Phylogenetic Conservation.
Article in Journal of extracellular vesicles, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed.
- Extracellular vesicles released by in vitro plant cell cultures: emerging systems for vesicle-mediated communication and biomedical translation.Plant cell reports · 2026Review
- Mechanisms and Translational Potential of Plant-Derived Extracellular Vesicles in Cardiovascular Disease.Cells · 2026Review
- Cross-stress memory in plants: mobile RNAs, extracellular vesicles, and local-to-systemic signal integration.Plant molecular biology · 2026Review
- Recent progress of plant-derived extracellular vesicle-like nanoparticles integrated with biomaterials for skin repair.Journal of nanobiotechnology · 2026Review
- Impact of drying on yield and composition of extracellular vesicles from Centella asiatica.Scientific reports · 2026Article
- Plant-Derived Nanovesicles: A Comprehensive Review from Isolation to Clinical Translation-Unlocking Natural Nanocarriers for Biomedical Applications.Biomolecules · 2026Review
- Mechanistic Insights into Plant-Derived Exosomes, Their Cross-Kingdom Effects, and Potential Biomedical Applications in Skin Wounds Repair.Plants (Basel, Switzerland) · 2026Review
- Environmental Regulation, Molecular Profiling, and Preliminary Functional Evaluation of Extracellular Vesicles fromFoods (Basel, Switzerland) · 2026Article
- Plant-derived nanocarriers & nanostructures for barrier-defined translation: a comprehensive review of mechanisms, targets, and translation.Journal of nanobiotechnology · 2026Review
- Microalgal Exosome-like Nanovesicles fromMarine drugs · 2026Article
- Comparative proteomics reveals protein signatures shared between and unique to bona fide plant EVs and other plant-derived vesicles.Plant physiology · 2026Article
- Flow Cytometry Role in Unlocking New Frontiers for Nanomedicine Applications of Plant-Derived Vesicles.Journal of extracellular biology · 2026Review
- Isolation and Functional Characterization of Carob-Derived Nanovesicles Reveals Anti-Inflammatory and Regenerative Potential.Plants (Basel, Switzerland) · 2026Article
- Characterization of Extracellular Vesicles from Cell Suspension Cultures ofPlants (Basel, Switzerland) · 2026Article
- Oral plant-derived exosome-like nanovesicles: a new therapeutic perspective for intestinal diseases.Frontiers in pharmacology · 2026Review
- Plant-derived extracellular vesicles for drug delivery: current and future.Regenerative biomaterials · 2026Review
- Dosing of plant-derived extracellular vesicles in traditional Chinese medicine: current status, root causes, and standardization pathways.Frontiers in pharmacology · 2026Article
- Plant-derived extracellular vesicles and nanoparticles: origins, functions, and applications.Frontiers in bioengineering and biotechnology · 2026Review
- Inhibitory Effect ofInternational journal of nanomedicine · 2026Article
- Isolation and Characterization of Extracellular Vesicles Derived from Mango Fruits.International journal of molecular sciences · 2025Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Although the field of plant EVs (PEVs) is experiencing exponential growth, rigorous characterisation complying with MISEV guidelines has not been yet implemented due to the lack of bona fide reference markers. In this work, we have paved the way for the standardisation of PEV markers, providing the most profound proteomic data so far from apoplastic washing fluid-EVs, a sample enriched in genuine extracellular vesicles from plant tissue of two reference plant species: Arabidopsis thaliana (Arath-EVs) and Brassica oleracea (Braol-EVs). Besides, we analysed the protein content of the soluble fraction of the apoplast and calculated the enrichment of the potential markers studied in EVs. Additionally, we have conducted an exhaustive analysis of the proteomic data available so far from genuine EVs from any plant species, evaluating current potential markers, together with those found in our proteomic analyses. Our results provide evidence supporting the potential use of the following families as PEV markers: aquaporins, vacuolar-type ATPase complex subunits, some fasciclin-like arabinogalactan proteins (FLAs), tetraspanins, syntaxins, germin-like proteins and calreticulins. Next, we analysed the presence of orthologues and their degree of conservation throughout plant taxa, as well as in 2 reference species from the animal kingdom: human and mouse. Their degree of conservation was compared with that of current animal EVs: CD63, CD81 and CD9. Among the protein families with potential to be used as PEV markers, 2 were found to be plant-specific: FLAs and germin-like proteins. On the other hand, aquaporins and vacuolar-type ATPase complex subunits showed the greatest degree of conservation across plant and animal kingdoms. Our results provide key insights on several aspects of classical and novel protein identity markers for PEVs to assist in the selection of the best candidates for standardisation: (1) species-specific abundance, (2) specificity for PEVs and (3) conservation and plant specificity.
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