Evidence map›Paper›PMID 40903803›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025

Stage-Resolved Phosphoproteomic Landscape of Mouse Spermiogenesis Reveals Key Kinase Signaling in Sperm Morphogenesis.

Tianyu Zhu, Yan Zhu, Xinyi Jiang, Xiangzheng Zhang, Bing Wang, Yu Chen, Yichen Zhao, Yulian Wang, Qi Zhou, Zhongyan Han and 16 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

26 authors.

Tianyu ZhuState Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Gusu School, Nanjing Medical University, Nanjing, 211166, China.
Yan ZhuState Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Gusu School, Nanjing Medical University, Nanjing, 211166, China.
Xinyi JiangState Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Gusu School, Nanjing Medical University, Nanjing, 211166, China.
Xiangzheng ZhangState Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Gusu School, Nanjing Medical University, Nanjing, 211166, China.
Bing WangDepartment of Bioinformatics, Fujian Key Laboratory of Medical Bioinformatics, School of Medical Technology and Engineering, Fujian Medical University, Fuzhou, 350122, China.
Yu ChenChangzhou Maternal and Child Health Care Hospital, Changzhou Medical Center, Nanjing Medical University, Changzhou, Jiangsu, 213000, China.
Yichen ZhaoState Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Gusu School, Nanjing Medical University, Nanjing, 211166, China.
Yulian WangState Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Gusu School, Nanjing Medical University, Nanjing, 211166, China.
Qi ZhouState Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Gusu School, Nanjing Medical University, Nanjing, 211166, China.
Zhongyan HanState Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Gusu School, Nanjing Medical University, Nanjing, 211166, China.
Yaling QiState Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Gusu School, Nanjing Medical University, Nanjing, 211166, China.
Mengjiao LuoState Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Gusu School, Nanjing Medical University, Nanjing, 211166, China.
Haixia TuState Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Gusu School, Nanjing Medical University, Nanjing, 211166, China.
Bingjie HaoState Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Gusu School, Nanjing Medical University, Nanjing, 211166, China.
Mengmeng GaoState Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Gusu School, Nanjing Medical University, Nanjing, 211166, China.
Jie RenState Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Gusu School, Nanjing Medical University, Nanjing, 211166, China.
Xin ZhouState Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Gusu School, Nanjing Medical University, Nanjing, 211166, China.
Xingyu ZhangState Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Gusu School, Nanjing Medical University, Nanjing, 211166, China.
Xu ChenState Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Gusu School, Nanjing Medical University, Nanjing, 211166, China.
Haojie LiState Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Gusu School, Nanjing Medical University, Nanjing, 211166, China.
Qian HuangState Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Gusu School, Nanjing Medical University, Nanjing, 211166, China.
Chenghao SituState Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Gusu School, Nanjing Medical University, Nanjing, 211166, China.
Yueshuai GuoState Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Gusu School, Nanjing Medical University, Nanjing, 211166, China.
Hui ZhuState Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Gusu School, Nanjing Medical University, Nanjing, 211166, China.
Yan LiDepartment of Clinical Laboratory, Sir Run Run Hospital, Nanjing Medical University, Nanjing, 211166, China.
Xuejiang GuoState Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, Gusu School, Nanjing Medical University, Nanjing, 211166, China.ORCID https://orcid.org/0000-0002-0475-5705

Funding

National Natural Science Foundation of China 82201764National Natural Science Foundation of China 82221005National Natural Science Foundation of China 82271636National Natural Science Foundation of China 82301808National Natural Science Foundation of China 82371606National Natural Science Foundation of China 82371623National Natural Science Foundation of China 82401873Natural Science Foundation of Jiangsu Province BK20220316
6 · The paper itself

Abstract

Spermiogenesis is the committed step of sperm production, during which spermatid cells undergo dramatic morphological transformations and transcriptional silencing. Post-translational modifications (PTMs), including phosphorylation, provide a level of protein function flexibility and play important roles in spermiogenesis. Dynamic protein phosphorylation profiles of spermatids are characterized across four different developing steps, and identified phosphorylation regulation of key proteins in spermiogenesis. Expression module and kinase-substrate phosphorylation network analysis revealed significant kinase activities of CSNK1G1 and TTBK2. CSNK1G1 is localized in the acrosome and is indispensable for acrosome biogenesis. Ttbk2 male germ cell conditional knockout mice are infertile with flagella development and head shaping defects. TTBK2 is essential for both the phosphorylation and stabilization of IFT88, an intraflagellar transport (IFT) protein with which TTBK2 colocalizes and interacts. Ciliogenesis defects in Ttbk2 knockout cells can be rescued by overexpression of TTBK2 or IFT88 but not kinase-dead TTBK2. Collectively, the systematic profiling of the spermiogenesis phosphoproteome revealed the dynamic nature and important functions of kinase phosphorylation in spermiogenesis and male fertility.

Indexed as

PhosphoproteinsSpermatogenesisSpermatozoaAnimalsMaleMiceMice, KnockoutPhosphorylationProtein Serine-Threonine KinasesProteomicsSignal TransductionSpermatidsPhosphoproteinsProtein Serine-Threonine KinasesCSNK1G1kinasephosphoproteomicsspermiogenesisTTBK2

Identifiers

PMID40903803
PMCPMC12667522

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.