ArticleBiological research2025
IL10 (-1082 G>A, rs1800896) gene polymorphisms are associated with oxidative stress in sickle cell disease patients in Uganda.
Article in Biological research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
backgroundSickle cell disease (SCD) is characterised by chronic oxidative stress. However, there is limited information on how polymorphisms in cytokine genes influence oxidative stress in SCD patients. The study aimed to determine the effect of Interleukin gene (IL-10) and Tumor Necrosis Factor (TNF
methodsA case control study with cross-sectional sample size of 163 SCD patients and 189 healthy controls was carried out. The extent of oxidative stress was quantified using Malondialdehyde (MDA) by spectrophotometry. Levels of IL-10 and TNF-α were measured using the Enzyme-Linked Immuno-Sorbent Assay (ELISA). The Amplification Refractory Mutation System polymerase chain reaction (ARMS-PCR) assay was used to genotype IL10-1082 A > G, (rs1800896), IL10-819 C > T (rs1800871) and TNF-α-308G > A (rs1800629) and TNF-β + 252 A > G (rs909253) gene polymorphisms.
resultsSamples showed significantly (P = 0.0063) higher median plasma levels of MDA in SCD patients (2.756µM) than healthy controls (2.364µM). A similar trend was observed with significantly (P < 0.0001) higher median plasma levels of IL-10 in SCD patients (20.37pg/ml) than healthy controls (7.5pg/ml). The most frequent genotype for IL-10 (-1082, rs1800896) gene polymorphism was heterozygous GA (62.6%). No significant association between IL10 (-1082G > A, rs1800896) gene polymorphisms and SCD was observed (OR = 1.08, 95% CI = 0.54-2.14, P = 0.87). Yet, IL10 homozygous GG (-1082, rs1800896) (22.12pg/ml) that was found to be significantly associated (P = 0.0234) with increased plasma levels of IL-10 as compared to heterozygous genotype (GA) (13.94pg/ml) in SCD patients. Similarly, higher levels of MDA were found to be significantly (P < 0.0001) associated with homozygous GG at IL-10 (-1082, rs1800896). The most frequent and only reported genotype for TNF-α/β gene polymorphisms were heterozygous GA, thus no associations were described.
conclusionIn conclusion, our results suggest that the IL10 (-1082 G > A, rs1800896) gene polymorphism is associated with increased oxidative stress and IL-10 cytokine level in Ugandan SCD patients.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.