Evidence map›Paper›PMID 40902823›Full record

ArticleNature2025

Cas9 senses CRISPR RNA abundance to regulate CRISPR spacer acquisition.

Xufei Zhou, Rucheng Diao, Xin Li, Christine A Ziegler, Max J Gramelspacher, Lydia Freddolino, Zhonggang Hou, Yan Zhang

Abstract read
In one paragraph

Article in Nature, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xufei Zhou *Department of Biological Chemistry, University of Michigan, Ann Arbor, MI, USA.ORCID 0000-0001-7119-8390
Rucheng Diao *Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI, USA.ORCID 0000-0002-2932-6755
Xin Li *Department of Biological Chemistry, University of Michigan, Ann Arbor, MI, USA.ORCID 0000-0002-1618-4786
Christine A ZieglerDepartment of Biological Chemistry, University of Michigan, Ann Arbor, MI, USA.ORCID 0000-0001-6982-8410
Max J GramelspacherDepartment of Biological Chemistry, University of Michigan, Ann Arbor, MI, USA.ORCID 0000-0003-3090-9314
Lydia FreddolinoDepartment of Biological Chemistry, University of Michigan, Ann Arbor, MI, USA. lydsf@umich.edu.ORCID 0000-0002-5821-4226
Zhonggang HouDepartment of Biological Chemistry, University of Michigan, Ann Arbor, MI, USA. houzg@umich.edu.ORCID 0000-0002-3676-6724
Yan ZhangDepartment of Biological Chemistry, University of Michigan, Ann Arbor, MI, USA. yzhangbc@med.umich.edu.ORCID 0000-0003-1678-5044

Funding

PREDOCTORAL TRAINING IN GENETICST32GM007544 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MORAN, JOHN V. · 1985 to 2022
$11.3M
Structure-based functional annotation of microbial genomesR01AI134678 · NIAID · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Lydia Petra Freddolino, SCOTT J. HULTGREN · 2018 to 2026
$6.5M
Building a unified framework for understanding bacterial gene regulation and chromosomal architectureR35GM128637 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Lydia Petra Freddolino · 2018 to 2026
$3.6M
Generation of DNA memory by bacterial CRISPR-Cas9 systemsR35GM137883 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Yan Zhang · 2020 to 2026
$3.0M
The alternative roles of Neisseria CRISPR-Cas9 in endogenous gene regulation and bacterial physiologyR00GM117268 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI ZHANG, YAN · 2017 to 2019
$747k
The alternative roles of Neisseria CRISPR-Cas9 in endogenous gene regulation and bacterial physiologyK99GM117268 · NIGMS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI ZHANG, YAN · 2016 to 2017
$104k
NIAID NIH HHS R01 AI134678NIGMS NIH HHS K99 GM117268NIGMS NIH HHS R00 GM117268NIGMS NIH HHS R35 GM128637NIGMS NIH HHS R35 GM137883NIGMS NIH HHS T32 GM007544
6 · The paper itself

Abstract

Prokaryotes create adaptive immune memories by acquiring foreign DNA snippets, known as spacers, into the CRISPR array

Indexed as

Clustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Associated Protein 9CRISPR-Cas SystemsAutoimmunityHumansRNA, Guide, CRISPR-Cas SystemsCRISPR-Associated Protein 9RNA, Guide, CRISPR-Cas Systems

Identifiers

PMID40902823
PMCPMC12477760

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.