Evidence map›Paper›PMID 40902591›Full record

ArticleCell reports methods2025

Feature-driven whole-tissue imaging with subcellular resolution.

Jinlong Lin, Zach Marin, Xiaoding Wang, Hazel M Borges, Qionghua Shen, Pierre-Emmanuel Y N'Guetta, Xuemei Luo, Baylee A Porter, Yuanyuan Xue, Md Torikul Islam and 11 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports methods, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

21 authors.

Jinlong LinLyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Cecil H. and Ida Green Center for Systems Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Zach MarinLyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Cecil H. and Ida Green Center for Systems Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Max Perutz Labs, Department of Structural and Computational Biology, University of Vienna, Vienna, VI 1030, Austria.
Xiaoding WangLyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Cecil H. and Ida Green Center for Systems Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Hazel M BorgesLyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Cecil H. and Ida Green Center for Systems Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Qionghua ShenLyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Cecil H. and Ida Green Center for Systems Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Pierre-Emmanuel Y N'GuettaDepartment of Cell Biology and Physiology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Xuemei LuoDepartment of Immunology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Baylee A PorterDepartment of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Yuanyuan XueChildren's Medical Center Research Institute at University of Texas Southwestern, Dallas, TX 75390, USA.
Md Torikul IslamChildren's Medical Center Research Institute at University of Texas Southwestern, Dallas, TX 75390, USA.
Tai NgoLyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Cecil H. and Ida Green Center for Systems Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Doreen IdonijeLyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Cecil H. and Ida Green Center for Systems Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Seweryn GałeckiLyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Cecil H. and Ida Green Center for Systems Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Department of Systems Biology and Engineering, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland.
Arin B AuroraDepartment of Radiation Oncology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Hu ZhaoChinese Institute for Brain Research, Beijing 102206, China.
Suzanne D ConzenDepartment of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Sean J MorrisonChildren's Medical Center Research Institute at University of Texas Southwestern, Dallas, TX 75390, USA; Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Shuang LiangDepartment of Immunology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Zhenyu ZhongDepartment of Immunology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Lori L O'BrienDepartment of Cell Biology and Physiology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Kevin M DeanLyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Cecil H. and Ida Green Center for Systems Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Electronic address: kevin.dean@utsouthwestern.edu.

Funding

UT Southwestern Medical Center Simmons Comprehensive Cancer CenterP30CA142543 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI Sandi Leigh Pruitt · 2010 to 2026
$53.7M
Estrogen and glucocorticoid receptor crosstalk in ER+ breastR01CA238519 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI CONZEN, SUZANNE DANIELA · 2019 to 2024
$1.7M
Translational Cancer Biology (TCB) T32 Training ProgramT32CA291654 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI Rolf A Brekken, Jerry William Shay · 2024 to 2026
$1.2M
NCI NIH HHS P30 CA142543NCI NIH HHS R01 CA238519NCI NIH HHS T32 CA291654
6 · The paper itself

Abstract

Existing microscopy approaches are often unable to identify and contextualize rare but biologically meaningful events due to limitations associated with simultaneously achieving both high-resolution imaging and a cm-scale field of view. Here, we present multiscale cleared tissue axially swept light-sheet microscopy (MCT-ASLM), a platform combining cm-scale imaging with targeted high-resolution interrogation of intact tissues in human-guided or autonomous modes. Capable of capturing fields of view up to 21 mm at micron-scale resolution, MCT-ASLM can seamlessly transition to a targeted imaging mode with an isotropic resolution that approaches ∼300 nm. This versatility enables detailed studies of hierarchical organization and spatially complex processes, including mapping neuronal circuits in rat brains, visualizing glomerular innervation in mouse kidneys, and examining metastatic tumor microenvironments. By bridging subcellular- to tissue-level scales, MCT-ASLM offers a powerful method for unraveling how local events contribute to global biological phenomena.

Indexed as

MicroscopyAnimalsBrainHumansKidneyMiceRatsTumor MicroenvironmentcancerCP: Imagingdevelopmentfeature-driven cleared tissue imagingLSFMmultiscale ASLMneuroscience

Identifiers

PMID40902591
PMCPMC12539259

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.