Evidence map›Paper›PMID 40901925›Full record

ArticleScience translational medicine2025

High-throughput synthesis and specificity characterization of natively paired influenza hemagglutinin antibodies with oPool

Wenhao O Ouyang, Huibin Lv, Wenkan Liu, Ruipeng Lei, Zongjun Mou, Tossapol Pholcharee, Logan Talmage, Meixuan Tong, Wei Ji, Yiquan Wang and 8 more

Abstract read
In one paragraph

Article in Science translational medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Molecular divergence and convergence of mammalian antibody responses to the influenza virus hemagglutinin stem.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

18 authors.

Wenhao O OuyangDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.ORCID 0000-0002-3618-9808
Huibin LvDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.ORCID 0000-0003-2218-0076
Wenkan LiuDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.ORCID 0009-0002-1702-3148
Ruipeng LeiDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.ORCID 0000-0002-4652-3400
Zongjun MouDepartment of Physiology and Biophysics, Case Western Reserve University, School of Medicine, Cleveland, OH 44106, USA.ORCID 0000-0002-1908-617X
Tossapol PholchareeDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Logan TalmageDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Meixuan TongDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Wei JiDepartment of Immunology and Microbiology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
Yiquan WangDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.ORCID 0000-0002-1954-9808
Katrine E DaileyDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Akshita B GopalDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Danbi ChoiDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Madison R ArdaghDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.ORCID 0009-0009-6935-9226
Lucia A RodriguezDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.ORCID 0009-0006-2872-4045
Jenna J GuthmillerDepartment of Immunology and Microbiology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.ORCID 0000-0001-7566-3536
Xinghong DaiDepartment of Physiology and Biophysics, Case Western Reserve University, School of Medicine, Cleveland, OH 44106, USA.ORCID 0000-0002-4102-3175
Nicholas C WuDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.ORCID 0000-0002-9078-6697

Funding

Sequence-function relationship of influenza broadly neutralizing antibodiesR01AI167910 · NIAID · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI Nicholas C. Wu · 2022 to 2026
$2.5M
High-throughput identification of antibody features for sequence-based epitope predictionDP2AT011966 · NCCIH · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI WU, NICHOLAS C. · 2021 to 2024
$2.4M
Shifting immunodominance of humoral immunity against influenza virusesDP2AI177692 · NIAID · UNIVERSITY OF COLORADO DENVER · PI Jenna Guthmiller · 2023 to 2026
$1.8M
NCCIH NIH HHS DP2 AT011966NIAID NIH HHS DP2 AI177692NIAID NIH HHS R01 AI167910
6 · The paper itself

Abstract

Antibody discovery is crucial for developing therapeutics and vaccines and for understanding adaptive immunity. However, the lack of approaches to synthesize antibodies with defined sequences in a high-throughput manner represents a major bottleneck in antibody discovery. Here, we present oPool

Indexed as

Antibodies, ViralAntibody SpecificityHemagglutinin Glycoproteins, Influenza VirusHigh-Throughput Screening AssaysHumansAntibodies, ViralHemagglutinin Glycoproteins, Influenza Virus

Identifiers

PMID40901925
PMCPMC12422562

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.