Evidence map›Paper›PMID 40901904›Full record

ArticlePlant biotechnology journal2025

JA Regulates Caffeine Biosynthesis in Tea Leaf for Resistance Against Fungal Infection and Antagonises With GA to Balance the Defence-Growth Trade-Off via CsDELLA-JAZ-MYC2-MYB184-TCS1 Module.

Zhili Ye, Ziqing Zheng, Jinsong Wang, Qinyu Zhong, Xiaoyu Mu, Zhoufang Yuan, Yin Huang, Zainab Ghazala, Hao Zuo, Yaling Li and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant biotechnology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Zhili YeState Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei, China.
Ziqing ZhengState Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei, China.
Jinsong WangState Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei, China.
Qinyu ZhongState Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei, China.
Xiaoyu MuState Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei, China.
Zhoufang YuanState Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei, China.
Yin HuangState Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei, China.
Zainab GhazalaState Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei, China.
Hao ZuoKey Laboratory of Tea Science of Ministry of Education, College of Horticulture, Hunan Agricultural University, Changsha, China.
Yaling LiState Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei, China.
Xiaoyu LiangState Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei, China.
Yujia QiState Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei, China.
Xiongyuan SiBiotechnology Center, Anhui Agricultural University, Hefei, China.
Yanrui ZhangState Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei, China.
Enhua XiaState Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei, China.
Jian ZhaoKey Laboratory of Tea Science of Ministry of Education, College of Horticulture, Hunan Agricultural University, Changsha, China.
Penghui LiState Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, Hefei, China.

Funding

Anhui Agricultural UniversityState Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilizationthe National Natural Science Foundation of China 32002089
6 · The paper itself

Abstract

Caffeine is a defensive alkaloid primarily accumulated in tea leaves to defend against pathogens. But the regulatory mechanism for caffeine biosynthesis in response to fungal infection and the trade-off between specialised metabolite and plant growth remain elusive. Here, we report that jasmonic acid (JA)-regulated CsMYB184 is the key for caffeine biosynthesis in tea leaves. Exogenous MeJA promoted caffeine biosynthesis in tea leaves and enhanced the resistance against fungal pathogens. JASMONATE ZIM-domain proteins (JAZs) interacted with CsMYB184 to repress CsMYB184 transactivation of TCS1. Meanwhile, JA effector CsMYC2 directly bound to and activated CsMYB184 transcription, but inhibited plant growth by directly modulating GA inactivation through gibberellin 2-oxidase 4 (CsGA2ox4). By contrast, gibberellins (GA) stimulated tea plant growth and inhibited caffeine biosynthesis, rendering tea plants more susceptible to fungal pathogens, via competitive DELLA proteins interacting with JAZs to relieve JAZ inhibition of CsMYB184. CsDELLA-JAZ-MYC2-MYB184-TCS1 formed an activation-repression loop to regulate developmental and hormonal control of caffeine biosynthesis in response to fungal pathogens, which balance the tea plant growth and defence response trade-offs. The study reveals JA-GA antagonistic regulation of caffeine biosynthesis as one of the innate immunity defences, meanwhile balancing the growth and defence of tea plants via crosstalk between JA and GA signalling.

Indexed as

CaffeineCamellia sinensisCyclopentanesGibberellinsOxylipinsPlant DiseasesDisease ResistanceGene Expression Regulation, PlantPlant Growth RegulatorsPlant LeavesPlant ProteinsCaffeineCyclopentanesGibberellinsjasmonic acidOxylipinsPlant Growth RegulatorsPlant Proteinsbiotic stresscaffeineCamellia sinensisphytohormone crosstalkresistancetranscriptional regulation

Identifiers

PMID40901904
PMCPMC12665076

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.