ArticlePloS one2025
Short-term effects of sweetened acidic beverages consumption on human saliva: Colloidal properties and protein composition.
Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Antioxidant and Cytoprotective Effects of Herbal Extract-Enriched Artificial Saliva: In-Vitro Biocompatibility and Cell Culture Studies.Oral health & preventive dentistry · 2026Article
- Sex-Specific Salivary Biochemical Composition in a Healthy Population.International journal of molecular sciences · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study investigates the impact of a sweetened acidic beverage, an apple juice (J) consumption on the tribological properties, viscoelasticity, and protein concentration/ composition of human saliva. Using a combination of tribological measurements, quartz crystal microbalance with dissipation monitoring (QCM-D), and protein analysis, we assessed how J may affect saliva's lubricating behaviour and adsorbed salivary film characteristics compared to water (control). Tribological results revealed that saliva (collected from 32 healthy adults) exposed to water or J exhibited increased friction when compared to unstimulated whole mouth saliva (uWMS), particularly within the boundary lubrication regime. A one-min rinse with water or J caused salivary delubrication, with water having a greater delubricating effect (p < 0.05) than that of J. Strikingly, the friction coefficients reverted to those for uWMS after 10 min (p > 0.05) highlighting the transient nature of delubrication caused by J consumption. This transient phenomenon was also evident in QCM-D measurements, where J transformed the ex vivo salivary film into a rigid layer, which reverted upon buffer application on model hydrophilic surfaces. Analysis of total protein concentration (TPC) showed that water significantly reduced TPC after one min, while J required 10 min to achieve similar TPC reduction. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) revealed that salivary cystatins and carbonic anhydrase significantly changed after J intervention, unlike water. This study highlights limited effects of J on salivary delubrication and adsorption. Building upon our findings, future research should investigate how repeated exposure to sweetened acidic beverages influences in vivo salivary pellicle dynamics and impacts to oral health.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.