ArticleReproductive medicine and biology
Sperm Injection at the Para-Polar Body Site in Piezo-Intracytoplasmic Sperm Injection Improves Subsequent Early Development of Bovine Embryos.
Article in Reproductive medicine and biology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Sperm Injection at the Para-Polar Body Site in Piezo-Intracytoplasmic Sperm Injection Improves Subsequent Early Development of Bovine Embryos.Reproductive medicine and biologyArticle
- Comment on "Sperm Injection at the Para-Polar Body Site in Piezo-Intracytoplasmic Sperm Injection Improves Subsequent Early Development of Bovine Embryos".Reproductive medicine and biologyArticle
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Authors and funding
4 authors.
Funding
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Abstract
Purpose: In piezo-ICSI, the first polar body (PB) of a metaphase II (MII) oocyte is generally oriented in the 6 or 12 o'clock position relative to sperm injection at 3 o'clock. However, the ooplasmic cell membrane may be damaged during drilling of the zona pellucida by piezo pulses. Here, we tested a new piezo-ICSI method in which the PB is set at the 2 or 4 o'clock position, so that zona drilling is performed through the widest position in the perivitelline space (para-PB piezo). Methods: The effect of piezo pulse position was evaluated by propidium iodide staining and cytoplasmic Ca Results: Piezo pulses may induce slight changes in the ooplasmic cell; our piezo-ICSI method can improve oocyte survival by minimizing damage to the ooplasmic cell membrane during zona drilling and consequently increasing the number of embryos available for transfer. Conclusions: Para-PB site piezo-ICSI is an improvement on current ICSI technology for animal and human reproduction.
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