ArticleFrontiers in public health2025
Next generation sequencing as a panacea for antibiotic susceptibility testing: yea or nay?
Article in Frontiers in public health, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Point of care antimicrobial susceptibility testing.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Practical next generation sequencing (NGS) technologies are entering the high-throughput diagnostic clinical microbiology laboratory. Bacterial whole genome sequences (WGS) can be used for detection and identification of species and their (relative) quantification. Genomic relatedness and epidemiological spread of strains of microorganisms can be traced, in parallel with detection of virulence genes as well as genes involved in antimicrobial resistance (AMR). The latter potentially facilitates genomic antimicrobial susceptibility testing (gAST). AMR mechanisms and the genes involved are diverse and require dedicated supporting databases in order to be accurately detected by microbial genomics. The present document assesses the current position of NGS and gAST assays in the clinical microbiology laboratory and discusses their role in establishing a clinically actionable antibiogram which defines the spectrum of antibiotics to which a given microbial strain is susceptible or resistant. Key question is whether or not gAST has added value as compared to current AST methodologies. Full diagnostic implementation of gAST in the routine medical microbiology laboratory is as yet impossible. The technical complexity of gAST still needs a significant decrease, gAST data management needs to be improved and simplified, the timeliness of the gAST assays requires improvement, and costs need to go down. The throughput of genomic testing for large-scale routine medical-microbiological testing needs to be enhanced. Its clinical value needs to be better defined and requirements for optimal market access and acceptance should be further developed. When forthcoming gAST has been shown to be compatible with insurance and reimbursement budgets as well as microbiological QA/QC assessment and has been through the European
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Registered trials
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