Evidence map›Paper›PMID 40900581›Full record

ArticleJournal of applied oral science : revista FOB2025

Etching on the edge: enamel loss under repeated and active HCl applications as a resin infiltration pretreatment.

Maria Paula Novaes Camargo Manna, Talita Portela Pereira, Bruna de Oliveira Iatarola, Mariele Vertuan, Ana Carolina Magalhães, Denise Maria Zezell, Luciana Fávaro Francisconi-Dos-Rios

Abstract readEvaluation Study
In one paragraph

Article in Journal of applied oral science : revista FOB, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. In Vitro Effects of Combining Resin Infiltration and At-Home Bleaching on Hydrogen Peroxide Penetration, Color Change and Enamel Morphology.Journal of esthetic and restorative dentistry : official publication of the American Academy of Esthetic Dentistry ... [et al.] · 2026
    Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Maria Paula Novaes Camargo MannaUniversidade de São Paulo, Faculdade de Odontologia, Departamento de Dentística, São Paulo, Brasil.ORCID http://orcid.org/0000-0002-9315-1507
Talita Portela PereiraInstituto de Ciência e Tecnologia, Faculdade de Odontologia, Departamento de Dentística, São José dos Campos (SP), Brasil.ORCID http://orcid.org/0000-0002-1402-0032
Bruna de Oliveira IatarolaUniversidade de São Paulo, Faculdade de Odontologia, Departamento de Dentística, São Paulo, Brasil.ORCID http://orcid.org/0000-0002-0413-0158
Mariele VertuanUniversidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Ciências Biológicas, São Paulo, Brasil.ORCID http://orcid.org/0000-0002-9254-3226
Ana Carolina MagalhãesUniversidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Ciências Biológicas, São Paulo, Brasil.ORCID http://orcid.org/0000-0002-6413-5348
Denise Maria ZezellUniversidade de São Paulo, Instituto de Pesquisas Energéticas e Nucleares, Centro de Lasers e Aplicações, São Paulo, Brasil.ORCID http://orcid.org/0000-0001-7404-9606
Luciana Fávaro Francisconi-Dos-RiosUniversidade de São Paulo, Faculdade de Odontologia, Departamento de Dentística, São Paulo, Brasil.ORCID http://orcid.org/0000-0002-9581-7544

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundConsidering that a single passive application of hydrochloric acid (HCl) as a resin infiltration pretreatment can remove between 20 and 45 µm of enamel and cause etching that extends up to 2 mm beyond the white spot lesions (WSLs), it is plausible that its repeated and active applications could result in a greater amount of dental tissue being removed.

objectiveTo evaluate the enamel surface loss and micromorphology after etching with 15% HCl using two application methods (passive-P and active-A) and varying numbers of applications (C-placebo - 120 s; 1x HCl - 120 s; 2x HCl - 120 s + 120 s; 3x HCl - 120 s + 120 s + 120 s). METHODOLOGY: Bovine incisors with ≤0.3 µm initial curvature were randomized into eight groups (n=12) based on microhardness, followed by WSL simulation. A central window was etched according to experimental conditions, and surface loss was assessed using optical profilometry and micromorphology via scanning electron microscopy (SEM). Two-way ANOVA and Tukey's test were used for surface loss, and the chi-square test evaluated the association of experimental conditions with etching patterns (α=0.05).

results1xP generated intermediate mean surface loss, positioned between the values observed for passive control (PC) and active control (AC), and those for 2xP and 3xP. Losses from active applications were significantly higher than passive ones and were increased by the number of applications. SEM showed Types II and III etching patterns and Type II was more frequent. There was no association between treatment and etching pattern.

conclusionMultiple and active HCl applications may raise concerns about removal of the remaining tooth structure, challenging the principles of minimal intervention dentistry.

Indexed as

Acid Etching, DentalDental EnamelHydrochloric AcidAnalysis of VarianceAnimalsCattleHardnessMaterials TestingMicroscopy, Electron, ScanningRandom AllocationReference ValuesReproducibility of ResultsResins, SyntheticSurface PropertiesTime FactorsTreatment OutcomeHydrochloric AcidResins, Synthetic

Identifiers

PMID40900581
PMCPMC12652427

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.