Evidence map›Paper›PMID 40900356›Full record

ArticleImmunologic research2025

Development of an in vitro method to assess the immunogenicity of biologics in the prevention of infectious diseases.

Joanna Baran, Łukasz Kuryk, Mariangela Garofalo, Marta Prygiel, Aleksandra Zasada, Teresa Szczepińska, Anna Staniszewska, Deepak Kala, Piotr Majewski, Radosław Charkiewicz and 1 more

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In one paragraph

Article in Immunologic research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Joanna BaranCentre for Advanced Materials and Technologies, Warsaw University of Technology, Warsaw, Poland.
Łukasz KurykNational Institute of Public Health NIH - National Research Institute, Warsaw, Poland.
Mariangela GarofaloDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, Padua, Italy.
Marta PrygielNational Institute of Public Health NIH - National Research Institute, Warsaw, Poland.
Aleksandra ZasadaNational Institute of Public Health NIH - National Research Institute, Warsaw, Poland.
Teresa SzczepińskaCentre for Advanced Materials and Technologies, Warsaw University of Technology, Warsaw, Poland.
Anna StaniszewskaMedical University of Lublin, Lublin, Poland.
Deepak KalaCENTERA Laboratory, Institute of High Pressure Physics, PAS, Warsaw, Poland.
Piotr MajewskiMedical University of Bialystok, Bialystok, Poland.
Radosław CharkiewiczMedical University of Bialystok, Bialystok, Poland.
Monika StaniszewskaCentre for Advanced Materials and Technologies, Warsaw University of Technology, Warsaw, Poland. monika.staniszewska@pw.edu.pl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We present a series of preclinical studies focused on developing in vitro 2D and 3D models for assessing immunogenic factors in preventing infectious diseases. Human peripheral blood mononuclear cells (PBMC) and Calu-3 cell lines (bronchial epithelial cells) were used to develop 2D and 3D models. Peptides: Spike-S1-His (S-His), nucleocapsid-His and adjuvants: human adenovirus five serotype-based viral vector (AdV-D24-ICOSL-CD40L), armed with inducible co-stimulator (ICOSL) and CD40 ligand (CD40L), and a vector lacking these transgenes (AdV5/3) were used due to their effective initial interaction with antigen-presenting cells (APC). Studying the potency of biologics in vitro revealed a significant increase in the percentage of CD4

Indexed as

Biological ProductsLeukocytes, MononuclearCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCell LineHumansBiological Products3D modelCalu-3CytokinesEx vivoImmunogenicityImmunologyInvivoLymphocytesSARS-CoV-2Vaccines

Identifiers

PMID40900356
PMCPMC12408726

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.