Evidence map›Paper›PMID 40900257›Full record

ArticleVeterinary research communications2025

Molecular detection and phylogenetic analysis of lumpy skin disease virus in Yunnan Province, China from 2019 to 2023.

Jun Yao, Xue Li, Lei Tan, Lin Gao, Pei Zhu, Jiarui Xie, Rui Yan, Taoyin Yu, Fengyi Xia, Qiyun Chen and 2 more

Abstract read
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In one paragraph

Article in Veterinary research communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Jun Yao *Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan Province, China. yaojun_joshua@hotmail.com.
Xue Li *College of Animal Medicine, Yunnan Agricultural University, Kunming, Yunnan Province, China.
Lei TanCollege of Animal Science and Technology, Yangtze University, Jingzhou, Hubei Province, China.
Lin GaoYunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan Province, China.
Pei ZhuYunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan Province, China.
Jiarui XieYunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan Province, China.
Rui YanAnimal Disease Prevention and Control Center of Menglian county, Puer, Yunnan Province, China.
Taoyin YuAnimal Disease Prevention and Control Center of Gongshan county, Nujiang, Yunnan Province, China.
Fengyi XiaAgricultural Comprehensive Service Center in Pengdang Township of Gongshan county, Nujiang, Yunnan Province, China.
Qiyun ChenAgricultural Vocational and Technical College of Yuxi City, Yuxi, Yunnan Province, China.
Bangquan ZengYunnan Animal Disease Prevention and Control Center, Kunming, Yunnan Province, China.
Jiaping YangAnimal Husbandry and Veterinary Medicine of Shidian county, Baoshan, Yunnan Province, China.

Funding

Innovation Guidance and Technology-Based Enterprise Cultivation Program of Yunnan Province Science and Technology Department 202304BI090003Key Research and Development Plan of Yunnan Province Science and Technology Department 202303AK140031
6 · The paper itself

Abstract

The lumpy skin disease (LSD), caused by the lumpy skin disease virus (LSDV), represents an emerging infectious disease that poses substantial economic losses to the cattle industries in China. This study aimed to investigate the epidemiological characteristics of LSDV in Yunnan Province, Southwest China, from 2019 to 2023. A Taqman-probe-based real-time PCR (qPCR) assay was developed for the molecular detection of LSDV nucleotides. In total, 2495 samples were collected and tested using the established method. LSDV-positive samples were further analyzed by amplifying and sequencing the GPCR and p32 genes. In addition, Viral isolation was performed to explore the biological characterisitics of the isolate. The developed qPCR assay demonstrated high sensitivity, with a limit of detection of 4.83 copies/µL. Six samples (0.24%) tested positive for LSDV, all originating from skin scabs. Molecular and phylogenetic analyses of the GPCR and p32 genes revealed that these six LSDV strains were genetically related to strains previously reported from China, Russia, and Thailand. One LSDV strain, designated YUN-LSDV, was successfully isolated in primary sheep testicular (PST) cells; while the isolate exhibited insensitivity to Madin-Darby bovine kidney cells. Collectively, this study represents the first report on the epidemiological characteristics of LSDV in cattle in Yunnan Province and contributes to the development of effective prevention and control strategies for LSD.

Indexed as

Lumpy Skin DiseaseLumpy skin disease virusAnimalsCattleChinaPhylogenyReal-Time Polymerase Chain ReactionGenetic and biologicalLumpy skin disease virusPhylogenetic analysisVirus isolationYunnan province

Identifiers

PMID40900257

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.