ArticleMicrobiology spectrum2025
Development and diagnostic performance of a sensitive multiplex pneumonia pathogen identification assay for emergency department patients with pneumonia.
Article in Microbiology spectrum, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Quantitative real-time PCR in the diagnosis of opportunistic infections in immunocompromised patients: Diagnostic performance, clinical utility, and future directions.Infectious diseases & immunity · 2026Review
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Authors and funding
12 authors.
Funding
Abstract
Pneumonia imposes a significant global health burden, with high morbidity and mortality, and challenges in pathogen identification due to diverse etiologies. Conventional culture-based methods are time-consuming and insufficient for fastidious organisms, necessitating advanced diagnostic tools for rapid and accurate pathogen identification, especially in emergency department (ED) settings. To address this, we developed the pneumonia pathogen identification (PPID) assay, a multiplex diagnostic platform based on the single-stranded multiplex PCR amplicons with suspension bead array technology. The PPID assay detects 27 bacterial and viral pathogens within 6 hours. Analytical validation using nucleic acids from reference strains or synthetic oligonucleotides demonstrated high specificity and sensitivity (5-500 copies per reaction). In 135 ED cases with pneumonia, the PPID assay significantly outperformed microbiological culture methods (77.8% vs 45.2%), increasing the overall detection rate to 85.9% when combined. It effectively identified polymicrobial infections in 48.6% of positive cases, including bacterial-viral co-infections, with
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