ArticleACS nano2025
Effects of Oligolysine-Polyethylene Glycol Coating on the Biodistribution of Wireframe DNA Origami Nanosheets in Zebrafish Embryos.
Article in ACS nano, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA origami-based nanotechnology is a versatile tool for exploring fundamental biological questions and holds significant promise for future biomedical applications. Here, we leverage the optical transparency of the embryonic zebrafish to analyze live embryos injected intravenously with fluorescently labeled wireframe DNA origami nanosheets. Our approach integrated long-term, high-resolution imaging of transgenic live zebrafish embryos with single-cell RNA sequencing to elucidate the effects of oligolysine-polyethylene glycol copolymer (K-PEG) coating on the biodistribution of fluorescence signal in embryos injected with wireframe DNA origami nanosheets. We observed rapid accumulation of fluorescence signal in the caudal hematopoietic tissue (CHT). K-PEG coating mitigated the accumulation of fluorescence signal in CHT, enabling increased detection of signal in other tissues. Our findings highlighted the pivotal role of scavenger endothelial cells in DNA origami clearance, with K-PEG enabling the prolonged detection of fluorescence signal at the CHT. Furthermore, using a transgenic zebrafish line designed for targeted macrophage ablation, we found that macrophages contribute to the clearance of fluorescence signal in embryos injected with the noncoated but not with K-PEG-coated nanosheets. This study introduces a framework for the analyses of the biodistribution and clearance of DNA origami nanostructures
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