ArticleJournal of the American Society for Mass Spectrometry2025
An Online Buffer Exchange Platform for Charge Detection Mass Spectrometry Analysis of AAVs and AAV-Antibody Complexes.
Article in Journal of the American Society for Mass Spectrometry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Native Multidimensional Protein Complex Identification and Topology Characterization (Native MudPIT).Analytical chemistry · 2026Article
- Deep Proteoform Sequencing with Top-Down Direct Mass Technology.bioRxiv : the preprint server for biology · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adeno-associated viruses (AAVs) are leading vectors in gene therapy that have demonstrated great potential in combating a wide range of human diseases. To enhance specificity and reduce dosing requirements, antibody-retargeted AAVs have emerged as a promising strategy to redirect vectors to novel receptors, thereby achieving improved efficacy and safety. However, effective characterization of AAVs and AAV-antibody complexes is complicated by heterogeneities that arise from variations in capsid protein assembly, genome integrity, and antibody decorations, demanding high-resolution techniques beyond traditional methods. Charge detection mass spectrometry (CDMS) is an emerging technique that effectively characterizes complex AAV samples by directly measuring ion charge. This technique is typically performed using static infusion-based nanoelectrospray ionization with long acquisition times, which is highly manual and subject to unstable spray and sample instability. To address limitations of static infusion in CDMS, we have developed a novel automated platform: size-exclusion chromatography-based flow injection coupled with CDMS (SEC-FI-CDMS). This platform streamlines and automates sample introduction with a dual-flow setup, enabling fast online desalting in high-flow mode and stable, prolonged infusion in low-flow mode for Orbitrap-based CDMS analysis. It accurately determines AAV genome packaging ratios and masses, differentiates capsid assembly and payload variations, and resolves antibody decoration on AAV-antibody complexes. This comprehensive characterization supports AAV manufacturing and development, paving the way for more effective next generation gene therapies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.