Evidence map›Paper›PMID 40898275›Full record

ArticleVirology journal2025

SDS-EDTA-treated chromatography paper strips for sampling and transportation of monkeypox virus, varicella zoster virus and herpes simplex 1 and 2 viruses.

Nikola Sklenovská, Mandy Bloemen, Graciela Andrei, Katrien Bruyninckx, Elke Wollants, Marc Van Ranst

Abstract readEvaluation Study
In one paragraph

Article in Virology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Nikola SklenovskáDepartment of Microbiology, Immunology and Transplantation, Laboratory of Clinical and Epidemiological Virology, KU Leuven, Leuven, 3000, Belgium. nikola.sklenovska@kuleuven.be.
Mandy BloemenDepartment of Microbiology, Immunology and Transplantation, Laboratory of Clinical and Epidemiological Virology, KU Leuven, Leuven, 3000, Belgium.
Graciela AndreiDepartment of Microbiology, Immunology and Transplantation, Molecular, Structural and Translational Virology Research Group, KU Leuven, Leuven, 3000, Belgium.
Katrien BruyninckxDepartment of Laboratory Medicine, University Hospitals Leuven, Leuven, 3000, Belgium.
Elke WollantsDepartment of Microbiology, Immunology and Transplantation, Laboratory of Clinical and Epidemiological Virology, KU Leuven, Leuven, 3000, Belgium.
Marc Van RanstDepartment of Microbiology, Immunology and Transplantation, Laboratory of Clinical and Epidemiological Virology, KU Leuven, Leuven, 3000, Belgium.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mpox is a viral zoonotic disease characterized by flu-like symptoms followed by rash, which can be difficult to distinguish from other rash-presenting infections. Laboratory diagnosis is essential for confirming monkeypox virus (MPXV) infection; However, challenges in specimen transport and storage in endemic regions, especially in rural areas with limited infrastructure, hinder timely diagnosis. This study evaluates the use of SDS-EDTA-treated chromatography paper strips for testing different viral dilutions of MPXV, varicella-zoster virus (VZV), herpes simplex viruses 1 (HSV-1) and 2 (HSV-2) under different storage durations and temperatures. The results showed that all viral dilutions could be reliably detected even after 120 days at 37 °C. Notably, MPXV concentrations as low as 2 copies/µL were detected, highlighting the clinical relevance of these strips. This method could enhance mpox testing in resource-limited areas by overcoming logistical barriers, offering a cost-effective and reliable alternative for specimen collection, transport and storage in regions affected by mpox outbreaks.

Indexed as

Herpesvirus 1, HumanHerpesvirus 2, HumanHerpesvirus 3, HumanMonkeypox virusMpox, MonkeypoxSpecimen HandlingChromatographyEdetic AcidHumansSodium Dodecyl SulfateTemperatureEdetic AcidSodium Dodecyl SulfateDiagnosisMonkeypox virusMpoxMPXVSampling

Identifiers

PMID40898275
PMCPMC12403532

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.