Evidence map›Paper›PMID 40898044›Full record

ArticleBMC genomic data2025

High-quality genome assembly and annotation of live animal vaccine bacteria strains in South Korea.

Yeonkyeong Lee, Jin-Ju Nah, Hyun-Ok Ku, Il Jang

Abstract read
In one paragraph

Article in BMC genomic data, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Yeonkyeong LeeVeterinary Drugs and Biologics Division, Animal and Plant Quarantine Agency, 177, Hyeoksin 8-ro, Gimcheon-si, Gyeongsangbuk-do, Korea.
Jin-Ju NahVeterinary Drugs and Biologics Division, Animal and Plant Quarantine Agency, 177, Hyeoksin 8-ro, Gimcheon-si, Gyeongsangbuk-do, Korea.
Hyun-Ok KuVeterinary Drugs and Biologics Division, Animal and Plant Quarantine Agency, 177, Hyeoksin 8-ro, Gimcheon-si, Gyeongsangbuk-do, Korea.
Il JangVeterinary Drugs and Biologics Division, Animal and Plant Quarantine Agency, 177, Hyeoksin 8-ro, Gimcheon-si, Gyeongsangbuk-do, Korea. moa305ho@korea.kr.

Funding

Animal and Plant Quarantine Agency B-1543073-2024-26-01
6 · The paper itself

Abstract

objectivesSelecting and managing suitable seed strains according to standard principles is a critical factor in manufacturing high-quality biological products. In this study, we determined the complete genomes of live animal vaccine bacterial strains used in South Korea and comprehensively analyzed their gene annotations. This detailed genomic analysis provides a robust foundation for identifying and verifying whether manufacturers utilize the same seed strains at the strain level. Furthermore, these data serve as a valuable reference to ensure consistent mass production and reliable quality control of biological products. DATA DESCRIPTION: Complete genome sequencing and annotation were performed on five bacterial strains (Erysipelothrix rhusiopathiae NL-11, Clostridium chauvoei B, Bordetella bronchiseptica 12-147-AFQ2, Bordetella bronchiseptica S-55, and Mycoplasmoides gallisepticum str. F) obtained directly from live attenuated vaccines. A hybrid genome assembly approach combining Illumina short reads (NovaSeq 6000) and Oxford Nanopore long reads (MinION Mk1B) was used to obtain complete, high-quality, gap-free, and well-annotated genome sequences. The genome sizes of E. rhusiopathiae NL-11, C. chauvoei B, B. bronchiseptica 12-147-AFQ2, B. bronchiseptica S-55, and M. gallisepticum str. F were 1.78, 2.40, 5.34, 5.34, and 0.95 Mb, respectively.

Indexed as

Bacterial VaccinesGenome, BacterialMolecular Sequence AnnotationAnimalsRepublic of KoreaVaccines, AttenuatedBacterial VaccinesVaccines, AttenuatedBordetella bronchisepticaClostridium chauvoeiErysipelothrix rhusiopathiaeHybrid assemblyLive attenuated vaccineMycoplasmoides gallisepticumSeed lot system

Identifiers

PMID40898044
PMCPMC12406338

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.