ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025
Engineered Fusion Enzyme-Mediated Non-Consecutive Cyclization-Glycosylation Enables Heterologous Synthesis of Antifungal Enfumafungin.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- Engineered Fusion Enzyme-Mediated Non-Consecutive Cyclization-Glycosylation Enables Heterologous Synthesis of Antifungal Enfumafungin.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
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12 authors.
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Abstract
Enfumafungin-type antibiotics, represented by enfumafungin and fuscoatroside, constitute a distinct class of fungi-derived fernane-type triterpenoids renowned for their potent antifungal activity. Notably, ibrexafungerp, a semi-chemically synthesized analogue of enfumafungin, has recently received approval as a novel antifungal drug. Thus, reconstituting the heterologous biosynthesis of enfumafungin holds great significance, as it offers a promising route for high-level production. Herein, the Aspergillus oryzae S184 chassis is first optimized. By deleting ku80 gene and refining counter-selection procedure, site-specific gene integration and substantially shortened the time required for selection marker recycling are significantly enhanced. Subsequently, an artificial biosynthetic pathway potentially involved in enfumafungin biosynthesis is successfully reconstructed. Crucially, the native terpene cyclase (TC)-glycosyltransferase (GT) fusion enzyme, EfuA, involved in enfumafungin biosynthesis, lost its functionality in A. oryzae. Conversely, a designed fusion enzyme EfuA
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