Evidence map›Paper›PMID 40896718›Full record

ArticleMethodsX2025

Rapid one-step CRISPR-cas vector assembly by isothermal spacer removal linearization and sequence-ligation independent cloning (ISRL-SLIC).

Claus Krogh Madsen, Tobias Hanak, Henrik Aronsson, Henrik Brinch-Pedersen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in MethodsX, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Claus Krogh MadsenCrop Genetics and Biotechnology, AU Flakkebjerg, Department of Agroecology, Aarhus University, Forsogsvej 1 4200 Slagelse, Denmark.
Tobias HanakCrop Genetics and Biotechnology, AU Flakkebjerg, Department of Agroecology, Aarhus University, Forsogsvej 1 4200 Slagelse, Denmark.
Henrik AronssonDepartment of Biological and Environmental Sciences, University of Gothenburg, Box 461 405 30 Gothenburg, Sweden.
Henrik Brinch-PedersenCrop Genetics and Biotechnology, AU Flakkebjerg, Department of Agroecology, Aarhus University, Forsogsvej 1 4200 Slagelse, Denmark.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

CRISPR-Cas genome editing is a powerful tool in various fields, but current cloning methods can be time-consuming due to the frequent use of intermediate entry vectors and multiple steps involving restriction enzymes and ligases. These multiple steps can create a bottleneck in CRISPR-Cas experiments. In response to this challenge, we propose a highly efficient streamlined approach, which enables simultaneous linearization of the acceptor plasmid and protospacer cloning in a single isothermal reaction. This eliminates the need for entry vectors, pre-linearization of vectors, and

Indexed as

CRISPR-CasLigation independentNew genomic techniquesProtospacerSequence independentSingle strand annealing

Identifiers

PMID40896718
PMCPMC12395069

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.