Evidence map›Paper›PMID 40896332›Full record

ArticleBiotechnology, biotechnological equipment2025

Simplified MS2 phage-like particle production including a novel maturation protein internal fusion affinity tag.

Enos C Kline, Rose Duong, Qin Wang, Barry R Lutz

Abstract read
In one paragraph

Article in Biotechnology, biotechnological equipment, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Enos C KlineDepartment of Bioengineering, University of Washington, Seattle, WA, USA.ORCID 0000-0002-3879-5289
Rose DuongDepartment of Bioengineering, University of Washington, Seattle, WA, USA.ORCID 0009-0003-3536-0903
Qin WangDepartment of Bioengineering, University of Washington, Seattle, WA, USA.ORCID 0000-0002-3978-7811
Barry R LutzDepartment of Bioengineering, University of Washington, Seattle, WA, USA.ORCID 0000-0003-4298-4901

Funding

V-OLA: point-of-care HIV viral load monitoring and drug resistance testingR01AI145486 · NIAID · UNIVERSITY OF WASHINGTON · PI Barry Ryan Lutz · 2019 to 2026
$5.6M
HIV-specific target capture and quantitative isothermal amplification for acute HIV diagnosis and treatment monitoringR33AI140460 · NIAID · UNIVERSITY OF WASHINGTON · PI LUTZ, BARRY RYAN · 2021 to 2022
$1.5M
NIAID NIH HHS R01 AI145486NIAID NIH HHS R33 AI140460
6 · The paper itself

Abstract

Phage-like particles (PLPs) are fabricated self-assembling nanoparticles derived from the structural elements of bacteriophages. These particles have biotechnological utility because of the ability to easily modify surface chemistry and compartmentalize nucleic acids or other materials. A consequential implementation of PLPs in diagnostics is as process controls in nucleic acid amplification tests, where control RNAs are packaged within the protein capsid and protected from degradation by RNases in the sample matrix. Key developments in PLP controls have enhanced the packing efficiency of RNAs into particles, reduced the complexity of their plasmid expression systems, and shifted purification from ultracentrifugation to affinity chromatography, producing progressively greater yields with higher purity. Expanding on prior improvements, this study establishes a revised set of plasmid vectors for

Indexed as

affinity chromatographyinternal Amplification Control (IAC)phage displayPhage-Like Particle (PLP)protein fusionVirus-Like Particle (VLP)

Identifiers

PMID40896332
PMCPMC12393069

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.