Evidence map›Paper›PMID 40895474›Full record

ArticleFrontiers in microbiology2025

Development of a yeast-based CRISPR genome editing system for feline coronavirus.

Xiaohu Zhang, Jingru Zhu, Di Zhang, Yueping Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Xiaohu Zhang *State Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Jingru Zhu *State Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Di ZhangState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.
Yueping ZhangState Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Feline infectious peritonitis (FIP), caused by feline coronavirus (FCoV), is a highly lethal disease characterized by systemic organ infection in cats. Current challenges of FIP include the absence of definitive diagnostic criteria, effective vaccines, and targeted therapies. Developing a robust genome editing toolkit is therefore critical to unraveling FCoV replication and pathogenesis mechanisms, elucidating viral protein functions, and identifying promising diagnostic and therapeutic targets. Methods: In this study, we developed a yeast-based CRISPR genome editing system compatible with a TAR-generated FCoV infectious clone, enabling gene deletion, gene insertion, and point mutation with high efficiencies and accuracies. Results and discussion: This system not only will contribute to a better understanding of the pathogenic mechanisms of FCoV but also serves as a valuable platform for vaccine development. Furthermore, it offers a possible strategy for genome editing and reverse genetics for other coronaviruses.

Indexed as

CRISPRfeline coronavirusfeline infectious peritonitisreverse geneticsTARvirus rescueyeast artificial chromosome

Identifiers

PMID40895474
PMCPMC12391068

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.