Evidence map›Paper›PMID 40895302›Full record

ArticleFrontiers in cellular and infection microbiology2025

IL-10/STAT5 axis suppresses miR-140 to upregulate B7-H4 expression in RAW264.7 cells.

Dandan Zhu, Guo Chen, Pei Shen, Weiliang Fan, Chuxin Ji, Yinong Duan, Wenxi Gao

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In one paragraph

Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Dandan Zhu *Department of Pathogen Biology, School of Medicine, Nantong University, Nantong, Jiangsu, China.
Guo Chen *Department of Pathogen Biology, School of Medicine, Nantong University, Nantong, Jiangsu, China.
Pei Shen *Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, China.
Weiliang FanDepartment of Pathogen Biology, School of Medicine, Nantong University, Nantong, Jiangsu, China.
Chuxin JiDepartment of Pathogen Biology, School of Medicine, Nantong University, Nantong, Jiangsu, China.
Yinong DuanDepartment of Pathogen Biology, School of Medicine, Nantong University, Nantong, Jiangsu, China.
Wenxi GaoDepartment of Pathogen Biology, School of Medicine, Nantong University, Nantong, Jiangsu, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Methods: Western blot was performed to detect B7-H4 expression levels, both in mice infected with Results: Notably, miR-140 was decreased in IL-10-treated microphages, accompanied by B7-H4 expression was upregulated. miR-140 can directly bind to the 3'UTR of B7-H4 and then inhibit the expression of B7-H4 in RAW264.7 cells. Meanwhile, miR-140 mimics can also attenuate IL-10-induced B7-H4 expression in RAW264.7 cells. Then we found that IL-10 may inhibit miR-140 promoter activity in RAW264.7 cells through transcription factors that binding to the - 576/- 94 bp region of the miR-140 promoter. Results by Western blot and ChIP further indicated that IL-10 could downregulate miR-140 promoter activity in a STAT5 dependence manner. After the sequence of STAT5 binding site within the - 456/- 446 bp region of the miR-140 promoter was mutated, IL-10 failed to suppress the activity produced by mutant miR-140 promoter. Discussion: In summary, IL-10 can inhibit miR-140 through STAT5, thereby upregulating the expression of B7-H4 in RAW264.7 cells. This study may suggest a new mechanism underlying IL-10-mediated B7-H4 upregulation in macrophages.

Indexed as

Interleukin-10MicroRNAsV-Set Domain-Containing T-Cell Activation Inhibitor 13' Untranslated RegionsAnimalsGene Expression RegulationMacrophagesMicePromoter Regions, GeneticRAW 264.7 CellsSchistosoma japonicumSchistosomiasis japonicaUp-Regulation3' Untranslated RegionsIL10 protein, mouseInterleukin-10MicroRNAsMIRN140 microRNA, mouseV-Set Domain-Containing T-Cell Activation Inhibitor 1B7-H4IL-10miR-140Schistosoma japonicumSTAT5

Identifiers

PMID40895302
PMCPMC12394516

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.