ArticlePeerJ2025
MultNAT HIV-1 Viral Load Assay: a simple, rapid and sensitive cross-primer amplification assay for detecting the HIV-1 virus in the field.
Article in PeerJ, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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9 authors.
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Abstract
Background: A large number of new human immunodeficiency virus (HIV) patients are diagnosed worldwide every year. This study aimed to design a rapid, efficient, and convenient kit for detecting HIV-1 viral load. Methods: The sensitivity and specificity of the kit were determined, and serum samples were collected from HIV-negative and HIV-positive individuals who underwent medical checkups at Xixi Hospital in Hangzhou City, Zhejiang Province, China, between June and August 2023 for testing. The presence of HIV-1 in the clinical samples was assessed Results: HIV-1 RNA was measured with a 100 copies/mL sensitivity Conclusion: Due to its simplicity, rapid response time, and high sensitivity and specificity, the MultNAT HIV-1 Viral Load Assay is suitable for primary care and epidemiological screening, facilitating rapid and accurate HIV-1 detection.
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