Evidence map›Paper›PMID 40895040›Full record

ArticlePeerJ2025

Reverse transcription recombinase polymerase amplification-lateral flow assay for detection of pathogenic orthoflaviviruses in mosquito vectors.

Parinda Thayanukul, Ronald Enrique Morales Vargas, Konkamon Sujijun, Pimchanok Khumpeera, Kittiya Suksawat, Nahallage Dona Asha Dilrukshi Wijegunawardana, Patsamon Rijiravanich, Werasak Surareungchai, Pattamaporn Kittayapong

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In one paragraph

Article in PeerJ, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Parinda ThayanukulDepartment of Biology, Faculty of Science, Mahidol University, Ratchathewi, Bangkok, Thailand.
Ronald Enrique Morales VargasCenter of Excellence for Vectors and Vector-Borne Diseases, Faculty of Science, Mahidol University, Salaya, Nakhon Pathom, Thailand.
Konkamon SujijunDepartment of Biology, Faculty of Science, Mahidol University, Ratchathewi, Bangkok, Thailand.
Pimchanok KhumpeeraDepartment of Biology, Faculty of Science, Mahidol University, Ratchathewi, Bangkok, Thailand.
Kittiya SuksawatDepartment of Biology, Faculty of Science, Mahidol University, Ratchathewi, Bangkok, Thailand.
Nahallage Dona Asha Dilrukshi WijegunawardanaDepartment of Bioprocess Technology, Faculty of Technology, Rajarata University of Sri Lanka, Mihintale, Sri Lanka.
Patsamon RijiravanichIndustrial Sensor Technology Research Team, National Center for Genetic Engineering and Biotechnology, National Sciences and Technology Development Agency at King Mongkut's University of Technology Thonburi, Bang Khun Thian, Bangkok, Thailand.
Werasak SurareungchaiNanoscience & Nanotechnology Graduate Programme, Faculty of Science, King Mongkut's University of Technology Thonburi, Bang Mot, Bangkok, Thailand.
Pattamaporn KittayapongCenter of Excellence for Vectors and Vector-Borne Diseases, Faculty of Science, Mahidol University, Salaya, Nakhon Pathom, Thailand.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: The genus Methods: Several previously published broad-specific primers targeting the genus Results: The primer FlaviPath-F and FlaviPath-R targeted 36% of the selected pathogenic orthoflavivirus species without cross-reacting with non-pathogenic strains based on Discussion: This is the first study to develop an RPA-based method for the detection of multiple orthoflavivirus pathogens in mosquito vectors. The reverse transcription recombinase polymerase amplification assays with lateral flow dipsticks (RT-RPA-LFD) platform offers a rapid, cost-effective tool for identifying regions at risk of arboviral transmission, supporting the targeting of individual viral diseases. This technique holds promise as an early warning system for emerging arboviral threats in public health.

Indexed as

FlaviviridaeMosquito VectorsNucleic Acid Amplification TechniquesAnimalsDNA PrimersHumansRecombinasesReverse TranscriptionRNA, ViralSensitivity and SpecificityZika VirusDNA PrimersRecombinasesRNA, ViralArbovirusBiosensorDip stripLateral Flow Detection (LFD)MosquitoMosquito-borne diseasesOrthoflavivirusRecombinase Polymerase Amplification (RPA)Reverse Transcription Recombinase Polymerase Amplification (RT-RPA)Vector

Identifiers

PMID40895040
PMCPMC12396212

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.