Evidence map›Paper›PMID 40894578›Full record

ArticlebioRxiv : the preprint server for biology2025

Endogenous Real Time Imaging Reveals Dynamic Chromosomal Mobility During Ligand-Mediated Transcriptional Burst Events.

Susan Wang, Thomas Suter, Amir Gamliel, Yeeun Kim, Sreejith J Nair, Soohwan Oh, Feng Yang, Kenneth A Ohgi, Tobias Wagner, Steven Gan and 1 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Susan WangDepartment and School of Medicine, University of California San Diego, La Jolla, CA 92093.
Thomas SuterDepartment and School of Medicine, University of California San Diego, La Jolla, CA 92093.
Amir GamlielDepartment and School of Medicine, University of California San Diego, La Jolla, CA 92093.
Yeeun KimBiomedical and Biological Sciences Graduate Program, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033.
Sreejith J NairDepartment and School of Medicine, University of California San Diego, La Jolla, CA 92093.
Soohwan OhDepartment and School of Medicine, University of California San Diego, La Jolla, CA 92093.
Feng YangDepartment and School of Medicine, University of California San Diego, La Jolla, CA 92093.
Kenneth A OhgiDepartment and School of Medicine, University of California San Diego, La Jolla, CA 92093.
Tobias WagnerDepartment and School of Medicine, University of California San Diego, La Jolla, CA 92093.
Steven GanBiological Sciences Graduate Program, University of California San Diego, La Jolla, CA 92093.
Michael G RosenfeldDepartment and School of Medicine, University of California San Diego, La Jolla, CA 92093.ORCID 0000-0002-1572-156X

Funding

HORMONAL AND DEVELOPMENTAL REGULATION OF GENE EXPRESSIONR01DK018477 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ROSENFELD, MICHAEL G · 1986 to 2025
$12.7M
U of Calif, San Diego Neuroscience Microscopy ImagingP30NS047101 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GLEESON, JOSEPH G, ZHENG, BINHAI · 2003 to 2022
$9.0M
TRAINING IN MOLECULAR &CELL BIOLOGY OF ALLERGYT32AI007469 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI BROIDE, DAVID H · 1994 to 2024
$6.9M
REGULATION OF NEUROENDOCRINE SWITCHING EVENTSR01DK039949 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ROSENFELD, MICHAEL G · 1996 to 2024
$2.4M
NIAID NIH HHS T32 AI007469NIDDK NIH HHS R01 DK018477NIDDK NIH HHS R01 DK039949NINDS NIH HHS P30 NS047101
6 · The paper itself

Abstract

Enhancers serve as the major genomic elements regulating mammalian signal-dependent transcriptional programs, characterized by alternating periods of target gene "bursting" and "non-busting" that require investigation of induced enhancer condensates and locus motility in real time to provide dynamic insights into signal/ligand-dependent regulatory events. Here, endogenous live cell imaging has revealed the altered chromosomal dynamics/condensate formation occurring during estrogen receptor α (ERα)-dependent target gene bursting/post-bursting and chronic activation events. Simultaneous DNA/RNA endogenous live imaging reveals that an increased mobility of acutely ERα-stimulated loci observed during the bursting phase is, unexpectedly, further increased in the subsequent non-burst phase. Single molecule tracking (SMT) of ERα shows that the relatively high-burst, lower-mobility acute state was indeed enriched for high-viscosity, 1,6-hexanediol-sensative ERα molecules in a low sub-diffusive confined state with enhanced condensate formation during burst activation. Consistent with this, blocking transcription with flavopiridol shifts DNA tracks into a non-confined state. Differential DNA kinetics during burst vs non-burst has provided a strategy to assess altered condensate formation during gene activation events. (165).

Identifiers

PMID40894578
PMCPMC12393356

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.