Evidence map›Paper›PMID 40894052›Full record

ArticleResearch square2025

Molecular identification of

Valerie T Nguyen, Renee L M N Ali, Bianca C Burini, Dalia S Dryden, Megan A Riddin, Kochelani Saili, Edgar Simulundu, Lawrence E Reeves, Douglas E Norris, Yoosook Lee

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Valerie T NguyenUniversity of Florida.
Renee L M N AliThe Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Johns Hopkins University.
Bianca C BuriniUniversity of Florida.
Dalia S DrydenUniversity of Florida.
Megan A RiddinUniversity of Pretoria.
Kochelani SailiMacha Research Trust.
Edgar SimulunduMacha Research Trust.
Lawrence E ReevesUniversity of Florida.
Douglas E NorrisThe Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Johns Hopkins University.
Yoosook LeeUniversity of Florida.

Funding

TransmissionU19AI089680 · NIAID · JOHNS HOPKINS UNIVERSITY · PI WILLIAM J MOSS · 2010 to 2026
$25.6M
Development of high-quality reference genomes for Anopheles squamosus and An. cydippisR03AI178041 · NIAID · UNIVERSITY OF FLORIDA · PI LEE, YOOSOOK · 2023 to 2024
$125k
NIAID NIH HHS R03 AI178041NIAID NIH HHS U19 AI089680
6 · The paper itself

Abstract

Background: Methods: Multiple alignments of previously published ITS2 contig sequences in NCBI from Results: A new assay consisting of a forward (ITS2-ASQ-R10, 5'-CCC TCG AAG GGT GCT GTG-3') and reverse (ITS2-ASQ-R10 5'-AAT CCA CGG TGT GAT GGC-3') primer reliably (> 94.8%) amplified an ITS2 fragment of 301bp length for Conclusions: The development of this robust PCR assay for

Indexed as

Anopheles squamosusinternal transcribed spacermalariamolecular diagnosismosquito

Identifiers

PMID40894052
PMCPMC12393599

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.