ArticleLasers in medical science2025
Determination of a safe protocol for using laser ablation with indocyanine green dye in endodontic treatment. In vitro, in vivo and human study.
Article in Lasers in medical science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study aimed to determine a safe protocol for using Laser Ablation therapy (LA) with Indocyanine Green (ICG) to treat infected root canals.
methodsIn vitro study - The root canal of an extracted human lower incisor was prepared to #35/.06 and was filled with 0.05% ICG dye. The λ 810 nm infrared diode laser was activated during the movement of introducing a #20 fiber into the root canals. The maximum temperature reached in the three root thirds was considered for protocols, modifying the power, pulse interval, and pulse duration. In vivo study - Extracted human lower incisor, lower premolar, and upper molar were selected. Their canals were prepared to #35/0.04, and the teeth were inserted into the subcutaneous tissue of Wistar rats. LA with ICG protocol selected in the in vitro study was performed on each root. The root surface temperature was measured in the three thirds. Then, the subcutaneous tissue adjacent to the roots was removed immediately or after 7 days and subjected to histological analysis in H&E stain. Human study - Thirteen lower incisors indicated for extraction from eleven patients were included. The canals of all teeth were prepared and received LA treatment with ICG in a similar way to the in vivo study. After tooth extraction, the apical thirds of the roots were processed for histopathological analysis.
resultsThe in vitro study revealed that the increase in power and pulse duration, or the reduction in pulse interval, determined the temperature rise. The protocol with the highest power that showed a temperature increase below 40 °C was the 2.5 W (power), 300 ms (interval), and 100 ms (duration). The in vivo study revealed that the greatest temperature variation was observed in the lower incisor; for this reason, it was selected for the human study. In the human study, a structure of the periodontal ligament with histological and morphological characteristics comparable to those of healthy tissues and similar to the roots of control teeth was observed in the treated teeth (p < 0.05). No harmful effects were observed on the cementum structure, periodontal ligament, and alveolar bone in any teeth evaluated.
conclusionsIt was concluded that the LA protocol used is safe and can be performed during the treatment of infected root canals.
Indexed as
Identifiers
40892096What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.