ArticleCancer medicine2025
Integrated Single-Cell RNA-Seq Reveals Immunosuppressive Mechanisms of Treg Cell Differentiation and Tumor Microenvironment Interactions in Colorectal Cancer.
Article in Cancer medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- KLRB1 gene in tumor immune regulation and disease prognosis: a multidimensional role review.Discover oncology · 2026Review
- TP53 Loss Fuels mTORC1 Activation and Autophagy Suppression to Drive Immune-Cold Colorectal Cancer.World journal of oncology · 2026Article
- Multi-omics analysis reveals that CAPG positive macrophages are key immunosuppressive drivers and prognostic determinants in the ferroptosis landscape of hepatocellular carcinoma.Discover oncology · 2026Article
- Reshaping the immunosuppressive glioma microenvironment: mechanisms, biomarkers, and emerging immunotherapies.Frontiers in immunology · 2026Review
- Integrated Single-Cell RNA-Seq Reveals Immunosuppressive Mechanisms of Treg Cell Differentiation and Tumor Microenvironment Interactions in Colorectal Cancer.Cancer medicine · 2025Article
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Authors and funding
6 authors.
Funding
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Abstract
backgroundColorectal cancer (CRC) remains a major challenge in oncology, necessitating the identification of new therapeutic targets. This study aimed to explore the CRC microenvironment and uncover potential targets using single-cell RNA sequencing (scRNA-seq).
methodsSingle-cell RNA sequencing data from GEO (GSE164522, GSE132465, and GSE144735) were integrated and stratified based on CRC clinical stages and tumor grades. Cell clustering analysis identified distinct cellular subsets, while survival and functional enrichment analyses evaluated the prognostic significance and biological activity of differentially expressed genes. Additionally, transcription factor activity and pseudotime trajectory analyses elucidated cellular dynamics during CRC progression. Cell-cell communication analysis identified key ligand-receptor interactions involved in immune regulation. Finally, multiplex immunofluorescence was used to validate the expression of key markers and cells in CRC tissues from patients.
resultsIn the low differentiated colorectal adenocarcinoma (LDCA) grade of CRC, the proportion of T cells significantly increased, accompanied by the enrichment of Treg cells and the upregulation of genes associated with immunosuppression, proliferation, and differentiation. Notably, TGFβ1
conclusionCollectively, TGFβ1
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Registered trials
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