Evidence map›Paper›PMID 40890574›Full record

ArticleBMC plant biology2025

Comprehensive analysis of RNA-seq data reveals novel insight in the formation of bamboo embryogenic callus in Bambusa changningensis.

Xiaoyan Gu, Zhou Fang, Jiarui Tan, Sen Chen, Jiahui Peng, Wei Feng, Yiqin Huang, Ying Cao, Shanglian Hu

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PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Xiaoyan Gu *Lab of Plant Cell Engineering, Southwest University of Science and Technology, Mianyang, 621010, Sichuan, China.
Zhou Fang *Lab of Plant Cell Engineering, Southwest University of Science and Technology, Mianyang, 621010, Sichuan, China.
Jiarui TanLab of Plant Cell Engineering, Southwest University of Science and Technology, Mianyang, 621010, Sichuan, China.
Sen ChenLab of Plant Cell Engineering, Southwest University of Science and Technology, Mianyang, 621010, Sichuan, China.
Jiahui PengLab of Plant Cell Engineering, Southwest University of Science and Technology, Mianyang, 621010, Sichuan, China.
Wei FengLab of Plant Cell Engineering, Southwest University of Science and Technology, Mianyang, 621010, Sichuan, China.
Yiqin HuangLab of Plant Cell Engineering, Southwest University of Science and Technology, Mianyang, 621010, Sichuan, China.
Ying CaoLab of Plant Cell Engineering, Southwest University of Science and Technology, Mianyang, 621010, Sichuan, China. caoying@swust.edu.cn.
Shanglian HuLab of Plant Cell Engineering, Southwest University of Science and Technology, Mianyang, 621010, Sichuan, China. shanglianhu1@163.com.

Funding

National Key R&D Program of China 2021YFD2200504_1 and 2021YFD2200505_2National Natural Science Foundation of China NSFC grant no. 32200270Ph.D. foundation from Southwest University of Science and Technology No. 22zx7147Science and Technology Project of Sichuan Province, China 2021YFYZ0006 and 2022NSFSC0093
6 · The paper itself

Abstract

Bamboo usually undergoes a prolonged vegetative growth period for several decades. Additionally, not all bamboo species produce seeds, and the regulatory mechanisms governing embryogenic callus formation remain unclear, which constrains molecular breeding progress in bamboo. Here, we used buds of Bambusa changningensis Yi et B. X. Li as explants for callus induction. The results revealed that bamboo embryogenic callus (EC) on media added coconut powder, 2,4-D and proline increased faster than that on other media. The content of EC increased with the increase of 2,4-D concentration on different media. The concentrations of auxin and gibberellin (GA) in non-embryogenic callus (NE) were significantly higher than those in EC. Furthermore, RNA-seq analysis revealed that 795 DEGs were mainly enriched in pathways of starch synthase and amyloplast metabolism. Gene set analysis revealed that genes associated with amylopectin synthesis and metabolism pathways were upregulated in EC, and several starch synthesis-related genes were significantly enriched in EC. We then observed that EC exhibited more clearly amyloplast, higher starch content, and three starch metabolism related genes (SS1, SBE1, STP-1) showed higher expression levels compared to NE. Collectively, these results identified that the transition from NE to EC was associated with amyloplast synthesis and metabolism, and it providing new insights into the establishment of callus regeneration and genetic transformation systems in bamboo.

Indexed as

BambusaGene Expression Regulation, PlantPlant Growth RegulatorsRNA-SeqSeedsStarchPlant Growth RegulatorsStarchAmyloplastBambooCallus regenerationEmbryogenic callus inductionGenetic transformationStarch synthase

Identifiers

PMID40890574
PMCPMC12400688

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