Evidence map›Paper›PMID 40888292›Full record

ArticleAnalytical chemistry2025

Bipartite Genetically Encoded Biosensors to Sense Calcium Ion Dynamics at Membrane-Membrane Contact Sites.

Issei Yamaguchi, Lucia Barazzuol, Giulia Dematteis, Wenchao Zhu, Yurong Wen, Mikhail Drobizhev, Dmitry Lim, Robert E Campbell, Tito Calì, Yusuke Nasu

Abstract read
In one paragraph

Article in Analytical chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Issei YamaguchiDepartment of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo113-0033, Japan.
Lucia BarazzuolDepartment of Biomedical Sciences, University of Padova, Padova 35131, Italy.
Giulia DematteisDepartment of Pharmaceutical Sciences, Università del Piemonte Orientale, Novara 28100, Italy.
Wenchao ZhuDepartment of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo113-0033, Japan.
Yurong WenCenter for Microbiome Research of Med-X Institute, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
Mikhail DrobizhevDepartment of Microbiology and Cell Biology, Montana State University, Bozeman, Montana 59717, United States.
Dmitry LimDepartment of Pharmaceutical Sciences, Università del Piemonte Orientale, Novara 28100, Italy.
Robert E CampbellDepartment of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo113-0033, Japan.ORCID 0000-0003-0604-092X
Tito CalìDepartment of Biomedical Sciences, University of Padova, Padova 35131, Italy.ORCID 0000-0002-8901-1659
Yusuke NasuDepartment of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo113-0033, Japan.ORCID 0000-0002-3193-9104

Funding

Resource for Multiphoton Characterization of Genetically-Encoded ProbesU24NS109107 · NINDS · MONTANA STATE UNIVERSITY - BOZEMAN · PI Mikhail Drobizhev · 2018 to 2026
$2.2M
NINDS NIH HHS U24 NS109107
6 · The paper itself

Abstract

Self-complementing bipartite fluorescent proteins (FPs) are useful tools for the detection of protein-protein proximity and for localizing fluorophores to membrane-membrane contact sites. Here, we report versions of circularly permuted green FP (GFP), red FP (RFP), and mNeonGreen (NG), which are split into a large fragment composed of nine β-strands and a small fragment composed of two β-strands. In each case, the large and small fragments can associate in live cells to form the complete 11-stranded FP β-barrel. We further converted each of these three self-complementing FPs into bipartite calcium ion (Ca

Indexed as

Biosensing TechniquesCalciumCell MembraneLuminescent ProteinsEndoplasmic ReticulumGreen Fluorescent ProteinsHeLa CellsHumansCalciumGreen Fluorescent ProteinsLuminescent Proteins

Identifiers

PMID40888292
PMCPMC12444748

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.