ArticleBMC oral health2025
Effects of injectable platelet-rich fibrin (i-PRF) application as root surface biomodification on gingival fibroblasts: an in vitro study.
Article in BMC oral health, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
backgroundRoot surface biomodification (RSB) enhances tissue attachment by removing the smear layer, facilitating collagen fibril formation, and promoting clot formation and stabilization. This study aimed to evaluate the efficacy of injectable platelet-rich fibrin (i-PRF), an autologous blood product, as a potential adjunct to ethylenediaminetetraacetic acid (EDTA) for RSB in gingival fibroblast attachment and proliferation in vitro.
methodsDentin discs (4 mm in diameter) underwent root surface debridement to remove damaged cementum. The discs were treated with 24% EDTA, followed by the application of i-PRF for 1, 3, 5-min. Enamel matrix derivatives (EMD) were used as a positive control (5 min application), while untreated discs served as the negative control. Cell viability was assessed using the XTT assay. Cellular morphology was examined via scanning electron microscopy (SEM), and cytoskeletal organization was analyzed using fluorescence microscopy (FM) analysis. Intracellular and extracellular alkaline phosphatase (iALP/eALP) gene expression levels were evaluated. Data was analyzed using ANOVA.
resultsXTT analysis revealed no statistically significant difference in cell viability between the i-PRF and the EMD groups at different time points. Actin filament organization was evident in the i-PRF 3-min group and became more pronounced in the i-PRF 5-min and the EMD groups. The i-PRF 3-min, i-PRF 5-min, and the EMD groups exhibited a spindle-shaped fibroblast morphology. No statistically significant difference was observed in iALP levels between the i-PRF 5-min and the EMD groups (p > 0.001). The EMD group exhibited the highest eALP level (p < 0.001), while the i-PRF 3-min and 5-min groups demonstrated the second highest levels.
conclusionThe application of i-PRF for 5 min following EDTA treatment appears to enhance gingival fibroblast proliferation, attachment, and ALP expression. The effectiveness of i-PRF may vary depending on the duration of the application. Therefore, in vitro data from i-PRF followed by EDTA application to dentin surfaces may be useful for further development of in vivo and clinical approaches.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.